KRIBIOLISA™ Protein A ELISA

Immunoassay for the measurement Natural & Structurally conserved Recombinant Protein A in samples containing Human immunoglobulin A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are generally purified through protein A column chromatography. This method of purification has the potential with contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody. The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as well as screening product prior to its use. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with anti- Protein A antibody coated on the microtitre plates. The wells are washed to remove any unbound reactants as per the wash procedure. Anti-Protein A HRP antibody is added to the wells and incubated. . The wells are washed to remove any unbound reactants as per the wash procedure. simultaneously in the microtiter wells already coated with affinity purified capture anti-Protein A antibody. The substrate 3,3’,5,5’ Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Protein A present.