KRIBIOLISA™ Human IgA ELISA

Immunoassay for quantitative determination of Human IgA in cell culture supernatant and other biological preparations Human IgA is an antibody playing a critical role in mucosal immunity, and found in the form of secretory IgA polymer in the secretions. Presence of such a trace contaminants in the products may lead to potential health hazards, especially when the product is intended for therapeutic use. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the Sandwich ELISA procedure. Samples containing IgA react with already coated affinity purified capture anti-Human IgA antibody and bind to them. Plates are washed with wash buffer to remove unbound reactants. Biotinylated Anti-Human IgA is added leading to formation of a sandwich complex of solid phase antibody-Human IgA-enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure. Streptavidin:HRP conjugate is added which binds to Biotinylated Anti- Human IgA complex. The wells are washed to remove any unbound reactants as per the wash procedure. The substrate 3, 3’,5, 5’ Tetra Methyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Human IgA present in the samples.