KRIBIOLISA™ FSH (Follicle Stimulating Hormone) Bioassay ELISA

Immunoassay for the quantification of hFSH in human Serum Human follicle stimulating hormone (FSH) is a glycoprotein hormone produced by the anterior pituitary gland. There are three other glycoprotein hormones, namely Thyroid Stimulating Hormone, Luteinizing Hormone (both produced by anterior pituitary gland) and Human Chorionic Gonadotropin (produced by the placenta) which are structurally similar. Each hormone has an alpha and beta subunit. The α subunits of each hormone are similar while the β subunit is specific to each hormone. The α subunits contain 92 amino acids while the β subunits vary with each hormone. The β subunit of both FSH and LH contain 115 amino acids, TSH 110 amino acids, and hCG 147 amino acids. The FSH and LH hormones function differently in females and males. It is to be noted that in women the growth and maturation of the ovarian follicle is dependent on FSH, while in men both LH and FSH act on the testes. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to FSH are pre- coated onto microwells. Samples and standards are pipetted into microwells and anti-FSH antibodies linked to HRP is added to the wells. FSH present in the sample and standards will bind to the plate and HRP Conjugate simultaneously. Washing is done to remove the unbound samples, standards and HRP conjugate. The ready to use substrate solution (TMB) is added to microwells and color develops directly proportional to the amount of FSH in the standards and sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.