KRIBIOLISA™ EPO (Erythropoietin) ELISA

Enzyme Immunoassay for the quantitative determination of EPO (Erythropoietin) in serum, plasma and cell culture supernatant Erythropoietin (EPO) is a heavily glycosylated protein with a molecular weight of about 30,000 - 34,000 Daltons. EPO is a polypeptide consisting of 166 amino acids, containing one O-linked and three N-linked carbohydrate chains. The recombinant EPO is a good substitute for the native protein for use in an immunoassay. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The method employs sandwich ELISA technique. Streptavidin is pre-coated onto microwells. Samples and standards are pipetted into microwells and EPO present in the sample are bound by the biotin labeled antibodies to the microwell. Anti-EPO-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of EPO in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.