Category: Molecular Biology Reagents

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  • Categories: Retrotranscriptases
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Reference: ISWAB‐DNA‐1200

DNA collection kit for humans that can capture samples producing high yields of double stranded, long fragmented DNA from buccal cells using traditional swabs.

Reference: RTM0301

The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.

Reference: R0424-01

The RNA-Lock Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, and white blood cells into the RNA-Lock Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at -20°C.

Reference: ISWAB‐DNA‐250

DNA collection kit for humans that can capture samples producing high yields of double stranded, long fragmented DNA from buccal cells using traditional swabs.

Reference: RTM0305

The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.

Reference: ev-8

1.25 µg pSpot8 vector, S.cerevisiae, Spot C-term, Leu/Amp (Ori: 2µ/pBR322), high expression.