Proteinase K Solution Reference: AC115 Proteinase K is a non-specific serine protease. This enzyme exhibits high stability and activity in the presence of SDS, EDTA, and urea, as well as over a wide pH range. Proteinase K is useful for the inactivation of nucleases and lysis during the isolation of DNA and RNA.
HighScriber™ Reverse Transcriptase Mix Reference: RTM0301 The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.
E.Z.N.A.® RNA-Lock Reagent Reference: R0424-01 The RNA-Lock Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, and white blood cells into the RNA-Lock Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at -20°C.
Proteinase K Solution Reference: AC116 Proteinase K is a non-specific serine protease. This enzyme exhibits high stability and activity in the presence of SDS, EDTA, and urea, as well as over a wide pH range. Proteinase K is useful for the inactivation of nucleases and lysis during the isolation of DNA and RNA.
HighScriber™ Reverse Transcriptase Mix Reference: RTM0305 The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.
E.Z.N.A.® RNA-Lock Reagent Reference: R0424-02 The RNA-Lock Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, and white blood cells into the RNA-Lock Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at -20°C.
RNase A Reference: AC117 For RNase digestion during DNA preparation. RNase A is endonuclease-free and has been rigorously tested for RNA digestion activity in plasmid purification procedures.
qScriber™ cDNA Synthesis Kit Reference: RTK0101 The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets. The highly active and thermostable HighScriber™ Reverse Transcriptase blended with RNase Inhibitor allows for an efficient cDNA synthesis and reaction safety. The wide reaction temperature range (38°C - 55°C) ensures efficient transcription from GC rich templates. The 5X qScriber™ Reaction Mix includes optimal concentrations of magnesium and dNTPs and a combination of anchored oligo (dT) and random hexamers for unbiased representation of mRNA ends. The kit is an optimal choice for generating high quality cDNA from viral RNA, miRNA or other targets for qPCR or for PCR. For the maximum convenience the Kit includes even the PCR Water to set up the reaction, so the only thing you need to take care of is the high quality RNA template.
E-Z 96 Plant RNA Kit Reference: R1027-01 Based on the same principle of E.Z.N.A.® Plant RNA Kit, the E-Z 96® Plant RNA kit is designed for isolation of plant RNA in a 96-well plate format. The E-Z 96® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. The kit includes a homogenization plate to efficiently remove cell debris and simultaneously homogenize the lysate. In combination with the HiBind RNA plate, this kit permits purification of high-quality RNA from as much as 40 mg seed tissue or up to 100 mg plant tissue. The system is also efficient enough to allow isolation of total RNA from as little as 0.5 mg tissue. Purified RNA is suitable for RT-PCR, Northern analysis, differential display, and poly A+ RNA selection.
RNase A Reference: AC118 For RNase digestion during DNA preparation. RNase A is endonuclease-free and has been rigorously tested for RNA digestion activity in plasmid purification procedures.
qScriber™ cDNA Synthesis Kit Reference: RTK0104 The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets. The highly active and thermostable HighScriber™ Reverse Transcriptase blended with RNase Inhibitor allows for an efficient cDNA synthesis and reaction safety. The wide reaction temperature range (38°C - 55°C) ensures efficient transcription from GC rich templates. The 5X qScriber™ Reaction Mix includes optimal concentrations of magnesium and dNTPs and a combination of anchored oligo (dT) and random hexamers for unbiased representation of mRNA ends. The kit is an optimal choice for generating high quality cDNA from viral RNA, miRNA or other targets for qPCR or for PCR. For the maximum convenience the Kit includes even the PCR Water to set up the reaction, so the only thing you need to take care of is the high quality RNA template.