Proteinase K Solution Reference: AC115 Proteinase K is a non-specific serine protease. This enzyme exhibits high stability and activity in the presence of SDS, EDTA, and urea, as well as over a wide pH range. Proteinase K is useful for the inactivation of nucleases and lysis during the isolation of DNA and RNA.
microLYSIS Reference: 2ML-50 Release buffer, optimised to lyse cells commonly used in the lab (like bacterial (e.g.E. coli), yeast (e.g.S. cerevisiae) and human cells (e.g. HeLa) for PCRready DNA in less than 15 minutes. Supplied in 1 tube for ease of use.
iSWAB DNA Collection Kit, 1.0ml Reference: ISWAB‐DNA‐1200 DNA collection kit for humans that can capture samples producing high yields of double stranded, long fragmented DNA from buccal cells using traditional swabs.
HighScriber™ Reverse Transcriptase Mix Reference: RTM0301 The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.
Proteinase K Solution Reference: AC116 Proteinase K is a non-specific serine protease. This enzyme exhibits high stability and activity in the presence of SDS, EDTA, and urea, as well as over a wide pH range. Proteinase K is useful for the inactivation of nucleases and lysis during the isolation of DNA and RNA.
microLYSIS Reference: 2ML-100 Release buffer, optimised to lyse cells commonly used in the lab (like bacterial (e.g.E. coli), yeast (e.g.S. cerevisiae) and human cells (e.g. HeLa) for PCR ready DNA in less than 15 minutes. Supplied in 1 tube for ease of use.
iSWAB DNA Collection Kit, 600ul Reference: ISWAB‐DNA‐250 DNA collection kit for humans that can capture samples producing high yields of double stranded, long fragmented DNA from buccal cells using traditional swabs.
HighScriber™ Reverse Transcriptase Mix Reference: RTM0305 The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.
RNase A Reference: AC117 For RNase digestion during DNA preparation. RNase A is endonuclease-free and has been rigorously tested for RNA digestion activity in plasmid purification procedures.
microLYSIS Reference: 2ML-250 Release buffer, optimised to lyse cells commonly used in the lab (like bacterial (e.g.E. coli), yeast (e.g.S. cerevisiae) and human cells (e.g. HeLa) for PCRready DNA in less than 15 minutes. Supplied in 1 tube for ease of use.
iSWAB RNA Collection Kit v2, 1.0ml Reference: ISWAB‐RNA‐v2 The iSWAB-RNA-v2 device allows for the non-invasive collection, concentration, and stabilization of intact buccal cells and/or any mammalian cells collected with a swab or cytobrush allowing for real time ambient stabilization of total RNA from the point of collection to processing. Maintaining RNA stability and protecting it from degradation is a significant challenge, while current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance.
qScriber™ cDNA Synthesis Kit Reference: RTK0101 The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets. The highly active and thermostable HighScriber™ Reverse Transcriptase blended with RNase Inhibitor allows for an efficient cDNA synthesis and reaction safety. The wide reaction temperature range (38°C - 55°C) ensures efficient transcription from GC rich templates. The 5X qScriber™ Reaction Mix includes optimal concentrations of magnesium and dNTPs and a combination of anchored oligo (dT) and random hexamers for unbiased representation of mRNA ends. The kit is an optimal choice for generating high quality cDNA from viral RNA, miRNA or other targets for qPCR or for PCR. For the maximum convenience the Kit includes even the PCR Water to set up the reaction, so the only thing you need to take care of is the high quality RNA template.