Category: Molecular Biology Reagents

Reference: DC105-01

FastPure FFPE DNA Isolation Kit is intended for isolating genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue, overcoming the inhibitory effect caused by formalin crosslinking. The deparaffinization solution in this kit is safer than xylene. The DNA-specific spin column and the unique buffer system also ensure the high quality of obtained DNA. The whole extraction process takes only 20 min (digestion time not counted). The extracted genomic DNA is of good integrity, high purity, and stable quality and can be used for a wide range of downstream applications, including PCR, qPCR, and library preparation.

Reference: GCOV9050

Reference: P505-d1

Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specific promotion factors and plateau release inhibitors. It has greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as lambda DNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using genomic DNA, etc. For complex templates, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can effectively amplify fragments up to 10 kb. Its mismatch rate is 1/53 of that of ordinary Taq enzyme, and it is Pfu enzyme 1/6. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added to inhibit at room temperature Its two monoclonal antibodies with 5'→3' polymerase activity and 3'→5' exonuclease activity can perform highly specific hot-start PCR. The amplified products are blunt-ended and are suitable for ClonExpress® fast Rapid cloning kit (C112/C113/C114). Application: Super fidelity: the fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. Every 300,000 bases amplified, with less than 5 mismatches; Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of cDNA can reach 10 kb, and the effective amplification length of genome can reach 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.

Reference: P505-d2

Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specific promotion factors and plateau release inhibitors. It has greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as lambda DNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using genomic DNA, etc. For complex templates, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can effectively amplify fragments up to 10 kb. Its mismatch rate is 1/53 of that of ordinary Taq enzyme, and it is Pfu enzyme 1/6. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added to inhibit at room temperature Its two monoclonal antibodies with 5'→3' polymerase activity and 3'→5' exonuclease activity can perform highly specific hot-start PCR. The amplified products are blunt-ended and are suitable for ClonExpress® fast Rapid cloning kit (C112/C113/C114). Application: The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; The optimized buffer system is widely applicable to various templates; Ready-to-use premix, minimizing experimental steps

Reference: P505-d3

Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specific promotion factors and plateau release inhibitors. It has greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as lambda DNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using genomic DNA, etc. For complex templates, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can effectively amplify fragments up to 10 kb. Its mismatch rate is 1/53 of that of ordinary Taq enzyme, and it is Pfu enzyme 1/6. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added to inhibit at room temperature Its two monoclonal antibodies with 5'→3' polymerase activity and 3'→5' exonuclease activity can perform highly specific hot-start PCR. The amplified products are blunt-ended and are suitable for ClonExpress® fast Rapid cloning kit (C112/C113/C114). Application: The fidelity is 52 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; The optimized buffer system is widely applicable to various templates; Ready-to-use premix, minimizing experimental steps

Reference: P515-01

Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specificity promoting factors, and plateau uninhibitory factors, which have greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Increased. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can Effectively amplify fragments up to 10 kb. The mismatch rate is 1/53 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added with two monoclonal antibodies that can inhibit its 5’→3’ polymerase activity and 3’→5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added in the system allows 2 × Master mix to maintain stable activity after repeated freezing and thawing. The amplified product is blunt-ended and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114).Application: Super fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are less than 5 mismatches; Amplify faster: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: simple templates such as plasmids and lambda DNA can be amplified up to 40 kb, cDNA can be amplified up to 10 kb, and genome can be amplified up to 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.

Reference: P515-02

Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specificity promoting factors, and plateau uninhibitory factors, which have greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Increased. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can Effectively amplify fragments up to 10 kb. The mismatch rate is 1/53 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added with two monoclonal antibodies that can inhibit its 5’→3’ polymerase activity and 3’→5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added in the system allows 2 × Master mix to maintain stable activity after repeated freezing and thawing. The amplified product is blunt-ended and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114).Application: Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches. Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of genome can reach 20 kb, and the effective amplification length of cDNA can reach 10 kb. Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples Simple operation: Dye premix, minimize experimental steps

Reference: P515-03

Phanta® Max Super-Fidelity DNA Polymerase is an upgraded version of Phanta® Super-Fidelity DNA Polymerase. Compared with the previous generation of products, Phanta® Max has added unique elongation factors, specificity promoting factors, and plateau uninhibitory factors, which have greatly improved the ability of long fragment amplification, amplification specificity and amplification yield. Increased. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can amplify fragments up to 20 kb; using cDNA templates, Phanta® Max can Effectively amplify fragments up to 10 kb. The mismatch rate is 1/53 of that of ordinary Taq enzyme and 1/6 of that of Pfu enzyme. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max is added with two monoclonal antibodies that can inhibit its 5’→3’ polymerase activity and 3’→5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added in the system allows 2 × Master mix to maintain stable activity after repeated freezing and thawing. The amplified product is blunt-ended and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114).Application: Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches. Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments. Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of genome can reach 20 kb, and the effective amplification length of cDNA can reach 10 kb. Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples. Simple operation: Dye premix, minimize experimental steps

Reference: P525-01

Phanta® Max has added unique elongation factors, specificity promoting factors and plateau uninhibiting factors, which greatly improves the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can effectively amplify fragments up to 20 kb; using cDNA templates, Phanta® Max It can effectively amplify fragments up to 10 kb. Its amplification mismatch rate is 1/53 of that of ordinary Taq polymerase and 1/6 of that of Pfu polymerase. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max contains two monoclonal antibodies that can inhibit its 5’ → 3’ polymerase activity and 3’ → 5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the system allows the 2 × Master mix to maintain stable activity after repeated freezing and thawing; the system contains an electrophoresis indicator, which can be directly spotted for electrophoresis after the PCR reaction is completed. The enzyme has 5'→ 3'polymerase activity and 3'→ 5'exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress® and topological cloning kits (C112/C113/C115/C601).Application: Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches. Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments. Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of genome can reach 20 kb, and the effective amplification length of cDNA can reach 10 kb. Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples. Simple operation: Dye premix, minimize experimental steps

Reference: P525-02

Phanta® Max has added unique elongation factors, specificity promoting factors and plateau uninhibiting factors, which greatly improves the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can effectively amplify fragments up to 20 kb; using cDNA templates, Phanta® Max It can effectively amplify fragments up to 10 kb. Its amplification mismatch rate is 1/53 of that of ordinary Taq polymerase and 1/6 of that of Pfu polymerase. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max contains two monoclonal antibodies that can inhibit its 5’ → 3’ polymerase activity and 3’ → 5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the system allows the 2 × Master mix to maintain stable activity after repeated freezing and thawing; the system contains an electrophoresis indicator, which can be directly spotted for electrophoresis after the PCR reaction is completed. The enzyme has 5'→ 3'polymerase activity and 3'→ 5'exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress® and topological cloning kits (C112/C113/C115/C601).Application: Super fidelity: the fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. Every 300,000 bases amplified, with less than 5 mismatches; Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments; Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of cDNA can reach 10 kb, and the effective amplification length of genome can reach 20 kb; Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.

Reference: P525-03

Phanta® Max has added unique elongation factors, specificity promoting factors and plateau uninhibiting factors, which greatly improves the ability of long fragment amplification, amplification specificity and amplification yield. Using simple templates such as λDNA and plasmids, Phanta® Max can effectively amplify fragments up to 40 kb; using complex templates such as genomic DNA, Phanta® Max can effectively amplify fragments up to 20 kb; using cDNA templates, Phanta® Max It can effectively amplify fragments up to 10 kb. Its amplification mismatch rate is 1/53 of that of ordinary Taq polymerase and 1/6 of that of Pfu polymerase. In addition, Phanta® Max has good resistance to PCR inhibitors and can be used for direct PCR of bacteria, fungi, plant tissues, animal tissues or whole blood samples. Phanta® Max contains two monoclonal antibodies that can inhibit its 5’ → 3’ polymerase activity and 3’ → 5’ exonuclease activity at room temperature, enabling highly specific hot-start PCR. This product contains Phanta® Max Super-Fidelity DNA Polymerase, dNTP and an optimized buffer system. It only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the system allows the 2 × Master mix to maintain stable activity after repeated freezing and thawing; the system contains an electrophoresis indicator, which can be directly spotted for electrophoresis after the PCR reaction is completed. The enzyme has 5'→ 3'polymerase activity and 3'→ 5'exonuclease activity, and the amplified product is blunt-ended, suitable for ClonExpress® and topological cloning kits (C112/C113/C115/C601).Application: Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches. Faster amplification: the limit speed can reach 0.5 sec/kb, 30 sec/kb can efficiently amplify most fragments. Longer amplification: the effective amplification length of simple templates such as plasmid and λ DNA can reach 40 kb, the effective amplification length of genome can reach 20 kb, and the effective amplification length of cDNA can reach 10 kb. Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples. Simple operation: Dye premix, minimize experimental steps

Reference: P510-01

2 × Phanta Flash Master Mix is based on the new generation superior enzyme Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Amplification will generate blunt-ended products, which are compatible with ClonExpress kits (Vazyme #C112/C113/C115) and TOPO cloning kit (Vazyme #C601)Application: This product is suitable for PCR with various templates such as genomic DNA, cDNA, plasmid DNA, dU-containing DNA, crude samples, etc.