ALLin™ Mega HS HiFi Red Mastermix Reference: HLM0505 highQu ALLin™ Mega HS HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. Compared to Mega HiFi, this hot start enzyme version allows for even higher sensitivity and specificity of PCR as well as for a room temperature reaction setup, and is excellent choice for multiplex reactions. The ALLin™ Mega HS HiFi DNA Polymerase is engineered to be much faster and to generate higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HS HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors. For maximum convenience, the ALLin™ Mega HS HiFi Mastermix, 2X (HLM0401) and ALLin™ Mega HS HiFi Red Mastermix, 2X (HLM0501) are available.
4X 1Step RT qPCR Probe ROX L Kit Reference: QOP0505 4X 1Step RT qPCR Probe Kits are designed for sensitive detection of specific RNAs, including virus RNA, in diluted high-volume samples. They combine a robust 4X qPCR mix (with Hot Start PCR Polymerase) with a 20X blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT4 Mix). This formulation allows for a high sample input volume with a reliable outcome of a single step RT qPCR when working with low copy number samples below 5 copies per reaction. 4X 1Step RT qPCR Probe Kits ensure the robust performance of both reverse transcription and qPCR reactions, which allows for the highest sensitivity viral RNA detection under fast qPCR cycling conditions. The performance of the kits has been tested for Sars-CoV-2 detection according to the recommended Charité Berlin protocol with appropriate primers/probes. PCR Water supplied in the kit ensures the best performance and reproducibility of the results. Depending on your instrument requirements, the kit is available as no ROX, ROX L (low) and ROX H (high) versions.
Genomic DNA Extracts – Specifity Standards for Lactobacillus acidophilus Reference: 51-1723 Lactobacillus acidophilus
5min TA/Blunt-Zero Cloning Kit Reference: C601-01 This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. The efficient connection is completed in 5 minutes at room temperature. Using a vector containing the suicide gene ccdB, when the insert is successfully connected to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thus achieving a "Zero" background. The addition of blunt-end factor makes this product compatible with TA cloning and blunt-end cloning.Application: Compatible: Compatible with TA cloning and blunt end cloning at the same time; Fast: add fragments and react for 5 minutes; High efficiency: high cloning efficiency and positive rate>95%.
PCR Enhancer Reference: P021-01 During conventional PCR analysis, DNA fragments with high GC content are often difficult to amplify due to their stable secondary structure. Under ordinary PCR reaction conditions, DNA polymerase is difficult or even impossible to amplify. It can intervene in the secondary structure of high GC content DNA. PCR Enhancer is a mixed additive composed of multiple components, which can effectively reduce the melting temperature of high GC templates and templates with complex secondary structures, and is compatible with almost all DNA polymerase amplification systems. When the optimized PCR program cannot effectively amplify the target fragment, adding PCR Enhancer can often get unexpected results. Application: Increase amplification stability; Improve amplification efficiency
E.Z.N.A.® Total RNA Kit II Reference: R6934-02 The E.Z.N.A.® Total RNA Kit II is designed for isolating total cellular RNA from tissues rich in fat, such as brain adipose tissues. However, this kit can also be used for the isolation of total RNA from other types of tissues including cultured eukaryotic cells, animal tissues, or bacteria. The E.Z.N.A.® Total RNA Kit II uses the reversible binding properties of HiBind® matrix, a new silica-based material. By combining the high lysis efficiency of RNA-Solv® Reagent with Omega Bio-tek's innovative HiBind® technology, this kit can extract total cellular RNA from all types of animal or human tissues including fatty tissues such as brain and adipose tissue. A specifically formulated high salt buffer system allows more than 100 µg RNA molecules greater than 200 bases to bind to the matrix. Cells or tissues are first homogenized with RNA-Solv® Reagent that inactivates RNases. After adding chloroform, the homogenate is separated into an aqueous and organic phase by centrifugation. The aqueous phase which contains the RNA is adjusted with ethanol and applied to the HiBind® RNA Mini Column to which total RNA binds, while cellular debris and other contaminants are washed away. High-quality RNA is eluted in Nuclease-Free Water.
E.Z.N.A.® Mollusc DNA Kit Reference: D3373-02 The E.Z.N.A.® Mollusc DNA Kit is designed for efficient purification of genomic DNA from molluscs, arthropods, round worms, flatworms and other invertebrate tissues rich in mucopolysaccharides. Fresh and frozen samples that have been preserved in alcohol or DNE can be used with this kit. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of HiBind matrix. Samples are homogenized and chloroform is added to remove mucopolysaccharides. Following a rapid alcohol precipitation step, DNA is further purified and all salts, proteins and other contaminants are removed. High-quality genomic DNA is suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
SampleIN™ Direct PCR Kit Reference: DPK0101 SampleIN™ Direct PCR Kit is a premium tool for a fast direct PCR eliminating the need of tedious template purification. The kit is excellent for direct PCR from mouse tail or ear, mammalian tissues, hair follicle, buccal swabs and blood. Rapid 15 min DNA extraction using DPK Lysis and Protease Buffers in a single tube generates PCR template extract which is further amplified under fast cycling conditions with a hot-start Taq master mix that includes red dye for direct gel loading. In a 2% agarose TAE gel the red dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The ALLin™ HS Red Taq Mastermix includes a hot start Taq DNA Polymerase what ensures high yield, specific, low background amplification. Mix components allow for a fast PCR cycling and increase success when working with complex templates or multiplexing. Generated A-tailed PCR products are suitable for ligating into TA cloning vectors, sequencing and other applications.
4X 1Step RT qPCR Probe ROX H Kit Reference: QOP0601 4X 1Step RT qPCR Probe Kits are designed for sensitive detection of specific RNAs, including virus RNA, in diluted high-volume samples. They combine a robust 4X qPCR mix (with Hot Start PCR Polymerase) with a 20X blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT4 Mix). This formulation allows for a high sample input volume with a reliable outcome of a single step RT qPCR when working with low copy number samples below 5 copies per reaction. 4X 1Step RT qPCR Probe Kits ensure the robust performance of both reverse transcription and qPCR reactions, which allows for the highest sensitivity viral RNA detection under fast qPCR cycling conditions. The performance of the kits has been tested for Sars-CoV-2 detection according to the recommended Charité Berlin protocol with appropriate primers/probes. PCR Water supplied in the kit ensures the best performance and reproducibility of the results. Depending on your instrument requirements, the kit is available as no ROX, ROX L (low) and ROX H (high) versions.