E.Z.N.A.® Insect DNA Kit Reference: D0926-02 The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
BetterBASE Reference: 3BBR25 Enhancing buffer designed for use with the ABI BigDye v1.1 and v3.1 sequencing mixes, for use with all sequencing machines replaces both home made and commercial dilution buffers, shown to improve results from difficult templates as well as reducing sequencing costs and is ideal for high throughput sequencing.
iSWAB RNA2 Collection Tube Rack 1.0ml x 50 Reference: ISR2‐T‐1200‐R The iSWAB-RNA-v2 device allows for the non-invasive collection, concentration, and stabilization of intact buccal cells and/or any mammalian cells collected with a swab or cytobrush allowing for real time ambient stabilization of total RNA from the point of collection to processing. Maintaining RNA stability and protecting it from degradation is a significant challenge, while current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance.
ALLin™ HS Red Taq Mastermix Reference: HSM0305 highQu ALLin™ Hot Start Taq DNA Polymerase is the superior sensitive enzyme. The activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of crude, complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Hot Start Taq DNA Polymerase is maximized by the use of 2X Red colored Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with PCR Water, and the only thing to add is the template with primers. ALLin™ HS Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. ALLin™ HS Red Taq Mastermix, 2X is also a key component in highQu SampleIN™ Direct PCR Kit (DPK0101/5), ensuring outstanding PCR results with crude samples.
ORA™ qPCR Green ROX L Mix Reference: QPD0105 highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Green Master mixes are available in different versions –with low or high ROX concentration.
CleanNGS Reference: CNGS-0050 The CleanNGS kit offers a highly efficient magnetic bead based clean-up system for the purification of both DNA and RNA for Next-Generation Sequencing and Sanger Sequencing workflows.
10CFU™ Sensitivity Standards for Mycoplasma citri Reference: 102-9003 Contains 3 vials with 10 CFU each and 2 negative controls. For the validation of detection limit and robustness, individual sample material according to EP 2.6.7 with inactivated mycoplasma particles.