HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) Reference: R312-02 HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.Application: Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product; Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns
Universal Loading Control Antibody Cocktail Reference: KTD101-EN_1 Kit Universal Loading Control Antibody Cocktail provides a comprehensive and detailed method to quickly and conveniently detect the content of load control proteins in a variety of samples.
E.Z.N.A.® Total RNA Midi Kit Reference: R6664-00 The E.Z.N.A.® Total RNA Midi Kit is designed for RNA isolation from large amounts of sample. The E.Z.N.A.® Total RNA Midi Kit can purify total RNA from up to 1x10^8 cultured cells or 200 mg animal tissues. By using the HiBind® midi column technology, the E.Z.N.A.® Total RNA Midi Kit greatly reduces processing time when compared to traditional methods such as CsCl ultracentrifugation or phenol/chloroform protocols. Purified RNA can be used in downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.
E.Z.N.A.® Insect DNA Kit Reference: D0926-00 The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
BetterBASE Reference: 3BBR-5 Enhancing buffer designed for use with the ABI BigDye v1.1 and v3.1 sequencing mixes, for use with all sequencing machines replaces both home made and commercial dilution buffers, shown to improve results from difficult templates as well as reducing sequencing costs and is ideal for high throughput sequencing.
iSWAB DNA Collection Tube Rack, 1.0ml x 50 Reference: ISD‐T‐1200‐R DNA collection kit for humans that can capture samples producing high yields of double stranded, long fragmented DNA from buccal cells using traditional swabs.
ALLin™ Hot Start Taq Mastermix Reference: HSM0205 highQu ALLin™ Hot Start Taq DNA Polymerase is the superior sensitive enzyme The activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Hot Start Taq DNA Polymerase is maximized by the use of 2X Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with PCR Water, and the only thing to add is the template with primers.
ORA™ SEE qPCR Probe ROX H Mix Reference: QPP0605 highQu qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimization. ORA™ SEE qPCR mixes provide an additional advantage of a simplified tracking of the process, as they are colored with an inert blue dye to make samples much better visible during pipetting and handling. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Probe Master mixes are available in three versions – without ROX, with low or high ROX concentration.
Clean Magnet plate 96-well Reference: CMAG-96-RN50 The Clean Magnet Plate 96-Well RN50 has been designed to accommodate a wide variety of applications.