Category: Molecular Biology Reagents

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Reference: R212-02

HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a special kit for cDNA one-strand synthesis that contains a genomic DNA removal step. The HiScript® II Reverse Transcriptase based on the Kit has a significant improvement compared with the previous generation HiScript® Reverse Transcriptase. Thermal stability, template affinity and inhibitor tolerance. The gDNA wiper used in the Kit can quickly remove genomic DNA contamination at 42°C for 2 minutes, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across exons. 10 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo dT23VN included in the kit has a stronger anchoring ability for Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo dT23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can synthesize full-length cDNA for cloning (up to 20 kb), but also synthesize cDNA with uniform reverse transcription efficiency for each position of qPCRApplication: Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 10 pg-5 μg total RNA, can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.; gDNA wiper Mix can quickly and completely remove genome contamination

Reference: CSB-CL015641HU1

Accesion number: BC009691

Reference: ev-31

10 µg pSpot Spot-Tag®-Actin vector for expression of Spot-Tag® β-actin fusion protein in mammalian cells - positive control.

Reference: BMU102-EN_100 mL

SuperKine™ West Femto Maximum Sensitivity Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments. In order to obtain the best experimental results, you need to optimize all your experimental factors, including the number of samples, antibody concentration, the use of membranes and blocking agents.

Reference: R1057-01

Omega Bio-tek's E.Z.N.A.® PX Blood RNA Kit is designed for isolation of total RNA from blood samples stored in special preserved reagents and Paxgene tubes. The procedure completely removes contaminants and enzyme inhibitors making RNA isolation fast, convenient, and reliable. RNA purified using the E.Z.N.A.® PX RNA Kit method is ready for applications such as RT-PCR.

Reference: 2MLP-2500

A more complex lysis solution that enables you to get intact DNA from the most difficult sources: The toughest bacterial cells and spores as well as yeasts, fungi, moulds, some plant and some animal tissues directly.

Reference: iSWAB‐MBF‐1200

iSWAB-Microbiome has been proven to efficiently inactivate bacteria, fungi, spores, and DNA/RNA viruses allowing safer transport of biological samples to processing labs, especially when samples are subject to long transit times. DNA and RNA from collected microorganisms remains stable at room temperature for up to 40 days for DNA, and 21 days for RNA, eliminating the need for expedited cold chain transport.