Exo-spin standard, 8 columns, 60 ml buffer Reference: EX01-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Blasticidin S, Powder Reference: BLA-1X Blasticidin S is used as a selection antibiotic that inhibits protein synthesis in bacteria and eukaryotes. Transformed cells containing the resistance genes bls, bsr, or BSD can be selected.
DMEM/Ham’s F-12, with L-Glutamine Reference: DMEM-12-A DMEM/Ham's F-12 is a widely used basal medium for supporting the growth of many different cell types. Due to the combination of components from DMEM and Ham’s F-12 it is a rich mixture.
Fetal Bovine Serum Advanced, Collected in South America Reference: FBS-11A Fetal Bovine Serum Advanced (FBS Advanced) has reduced batch-to-batch variations compared to standard FBS. It is suitable for the in vitro cultivation of numerous cell types.
Exo-spin standard, 24 columns, 250 ml buffer Reference: EX01-25 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Blasticidin S, Powder Reference: BLA-5X Blasticidin S is used as a selection antibiotic that inhibits protein synthesis in bacteria and eukaryotes. Transformed cells containing the resistance genes bls, bsr, or BSD can be selected.
DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES Reference: DMEM-12-HXA DMEM/Ham's F-12 is a widely used basal medium for supporting the growth of many different cell types. Due to the combination of components from DMEM and Ham’s F-12 it is a rich mixture.
Fetal Bovine Serum Advanced, Collected in South America Reference: FBS-11B Fetal Bovine Serum Advanced (FBS Advanced) has reduced batch-to-batch variations compared to standard FBS. It is suitable for the in vitro cultivation of numerous cell types.
Exo-spin standard, 24 columns, 500 ml buffer Reference: EX01-25L Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
G-418 Sulfate, Solution, 100 % Activity (50 mg/ml) Reference: G418-B G-418 Solution is used as a selection antibiotic in cell culture. It blocks polypeptide synthesis. Transformed cells containing the neomycin resistance genes neo can be selected.
DMEM High Glucose (4.5 g/l), with L-Glutamine Reference: DMEM-HA Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Fetal Bovine Serum, Collected in South America Reference: FBS-12A Fetal Bovine Serum (FBS) is the most frequently used growth supplement for cell culture media. High levels of embryonic growth promoting factors improve various cell culture applications.