Exo-spin standard, 8 columns, 60 ml buffer Reference: EX01-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
cipTEC 14.4 primary cells Reference: ciPTEC14.4 CiPTEC 14.4 is a human conditionally immortalized proximal tubular epithelial cell line. Stably transfected by SV40T and hTERT, subcloned and well-characterized. Expressing proximal tubular markers alkaline phosphatase, di-peptidiyl peptidase IV and ZO-1.
Blasticidin S, Powder Reference: BLA-1X Blasticidin S is used as a selection antibiotic that inhibits protein synthesis in bacteria and eukaryotes. Transformed cells containing the resistance genes bls, bsr, or BSD can be selected.
AmnioPrime Reference: APR-B AmnioPrime is a complete ready to use medium for amniotic fluid and chorionic villi cells. It is used for karyograms, fluorescence in situ hybridization and other cytogenetic methods.
DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium... Reference: DMEM-HA-P10 Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Hanks’ Balanced Salts, with Ca & Mg, w/o Phenol Red Reference: HBSS-1A Hanks' Balanced Salt Solution (HBSS) is a buffered salt solution. It is used in a variety of cell culture applications such as washing and transportation of cells or tissue preparation.
Exo-spin standard, 24 columns, 250 ml buffer Reference: EX01-25 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
cipTEC OAT 1 Reference: cipTECOAT1 Based on ciPTEC 14.4, including expression and functional uptake via organic anion transporter 1 (SLC22A6)
Blasticidin S, Powder Reference: BLA-5X Blasticidin S is used as a selection antibiotic that inhibits protein synthesis in bacteria and eukaryotes. Transformed cells containing the resistance genes bls, bsr, or BSD can be selected.
LymphoPrime Reference: LPR-B LymphoPrime is a complete ready to use medium for peripheral blood lymphocytes. It is mainly used for karyotyping and other cytogenetic studies.
DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium... Reference: DMEM-HA-P50 Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Hanks’ Balanced Salts, w/o Ca & Mg, w/o Phenol Red Reference: HBSS-2A Hanks' Balanced Salt Solution (HBSS) is a buffered salt solution. It is used in a variety of cell culture applications such as washing and transportation of cells or tissue preparation.