Exo-spin standard, 8 columns, 60 ml buffer Reference: EX01-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
cipTEC 14.4 primary cells Reference: ciPTEC14.4 CiPTEC 14.4 is a human conditionally immortalized proximal tubular epithelial cell line. Stably transfected by SV40T and hTERT, subcloned and well-characterized. Expressing proximal tubular markers alkaline phosphatase, di-peptidiyl peptidase IV and ZO-1.
DMEM/Ham’s F-12, with L-Glutamine Reference: DMEM-12-A DMEM/Ham's F-12 is a widely used basal medium for supporting the growth of many different cell types. Due to the combination of components from DMEM and Ham’s F-12 it is a rich mixture.
Hanks’ Balanced Salts, with Ca & Mg, w/o Phenol Red Reference: HBSS-1A Hanks' Balanced Salt Solution (HBSS) is a buffered salt solution. It is used in a variety of cell culture applications such as washing and transportation of cells or tissue preparation.
Adult Bovine Serum Reference: ABS-1A Adult Bovine Serum (ABS), collected from adult cattle, is used to supplement cell culture media. It is highly suitable for in vitro cultivation of multiple cell types.
Exo-spin standard, 24 columns, 250 ml buffer Reference: EX01-25 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
cipTEC OAT 1 Reference: cipTECOAT1 Based on ciPTEC 14.4, including expression and functional uptake via organic anion transporter 1 (SLC22A6)
DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES Reference: DMEM-12-HXA DMEM/Ham's F-12 is a widely used basal medium for supporting the growth of many different cell types. Due to the combination of components from DMEM and Ham’s F-12 it is a rich mixture.
Hanks’ Balanced Salts, w/o Ca & Mg, w/o Phenol Red Reference: HBSS-2A Hanks' Balanced Salt Solution (HBSS) is a buffered salt solution. It is used in a variety of cell culture applications such as washing and transportation of cells or tissue preparation.
Adult Bovine Serum Reference: ABS-1B Adult Bovine Serum (ABS), collected from adult cattle, is used to supplement cell culture media. It is highly suitable for in vitro cultivation of multiple cell types.
Exo-spin standard, 24 columns, 500 ml buffer Reference: EX01-25L Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.