Exo-spin standard, 8 columns, 60 ml buffer Reference: EX01-8 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Sodium Pyruvate (100 mM) Reference: NPY-B Sodium Pyruvate is a supplement for cell culture media. It is commonly used as a carbon source in addition to glucose in a final concentration of 1mM.
DMEM/Ham’s F-12, with L-Glutamine Reference: DMEM-12-A DMEM/Ham's F-12 is a widely used basal medium for supporting the growth of many different cell types. Due to the combination of components from DMEM and Ham’s F-12 it is a rich mixture.
Exo-spin standard, 24 columns, 250 ml buffer Reference: EX01-25 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
L-Glutamine (200 mM) Reference: GLN-B L-Glutamine is an essential amino acid usually supplemented to cell culture media. This solution is suitable for many cell culture applications.
DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES Reference: DMEM-12-HXA DMEM/Ham's F-12 is a widely used basal medium for supporting the growth of many different cell types. Due to the combination of components from DMEM and Ham’s F-12 it is a rich mixture.
Exo-spin standard, 24 columns, 500 ml buffer Reference: EX01-25L Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
Stable Glutamine (200 mM) Reference: STA-B Stable Glutamine (also known as L-Alanyl-L-Glutamine) is a direct substitute for L-Glutamine. This amino acid with increased stability is usually supplemented to cell culture media.
DMEM High Glucose (4.5 g/l), with L-Glutamine Reference: DMEM-HA Dulbecco's Modified Eagle Medium (DMEM) is a standard cell culture medium. It supports the growth of numerous cell types. The variety of available formulations suit many applications.
Exo-spin standard, 48 columns, 500 ml buffer Reference: EX01-50 Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), providing flexibility and performance. Using only precipitation for exosome isolation co-purifies large amounts of non-exosomal proteins and other material, as well as carried-over precipitant. SEC is reliable for exosome isolation, but a step is needed for low-exosome content starting material (such as cell culture media) to concentrate the sample prior to SEC isolation. For most applications, precipitation is the simplest way to achieve this concentration. Small samples can be purified directly. For larger samples we provide a simple, two-step protocol that allows consistent and reliable purification of samples in under 2 hours. Iterative loading can also be used to increase loading sample volume.
NCS21 Supplement (50x), Serum-free Reference: C21-H NCS21 Supplement is an optimized solution for serum-free cell culture applications. It is mostly used for cultivation of neuronal cell types.