PDS Kit, EBSS Vial Reference: LK003188 Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.
Cell Isolation Optimizing System Reference: LK003200 A complete method development kit containing an assortment of enzymes most frequently used in tissue dissociation and cell isolation procedures. Includes instructions, references and strategies for the handling, use and optimization of enzymatic cell isolation methods to achieve maximum yield of viable cells. Kit includes 500 mg of each of four types of collagenase, 500 mg trypsin, 50 ku hyaluronidase, 100 mg elastase, 100 mg papain, 25 mg DNase I, 10 mg neutral protease (Dispase®) and 100 mg trypsin inhibitor.
HBSS Solution Reference: LK003210 Sterile calcium and magnesium free Hank's balanced salt solution (CMFHBSS), pH 7.4, as supplied in the NCIS kit; 1 x 500 ml.
Trypsin Vial, NCIS Reference: LK003220 A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 2 ml of HBSS yields a solution of 500 µg/ml of trypsin, Code: TRLS. Suitable for cell isolation and culture applications.
Trypsin Vial, NCIS Reference: LK003225 A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 2 ml of HBSS yields a solution of 500 µg/ml of trypsin, Code: TRLS. Suitable for cell isolation and culture applications.
Inhibitor Vial, NCIS Reference: LK003230 A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 1 ml of HBSS or equivalent yields a solution of 2 mg/ml of trypsin inhibitor, Code: SIC. Suitable for cell isolation and culture applications.
Inhibitor Vial, NCIS Reference: LK003235 A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 1 ml of HBSS or equivalent yields a solution of 2 mg/ml of trypsin inhibitor, Code: SIC. Suitable for cell isolation and culture applications.
Collagenase Vial, NCIS Reference: LK003240 A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of HBSS or equivalent yields a solution of 300 units/ml of collagenase, Code: CLSPA. Suitable for cell isolation and culture applications.
Collagenase Vial, NCIS Reference: LK003245 A component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of HBSS or equivalent yields a solution of 300 units/ml of collagenase, Code: CLSPA. Suitable for cell isolation and culture applications.
L-15 Media Powder (L15NK) Reference: LK003250 Leibovitz L-15 media powder, a component of the NCIS kit. Reconstitute entire contents of pouch, QS to 1 liter with cell culture grade water, and 0.22 micron filter. Suitable for cell isolation and culture applications.
Cell Strainers (Falcon) Reference: LK003265 Cell strainers (Falcon), components of the NCIS kit. Suitable for removal of tissue debris in cell isolation applications.
Neonatal Cardiomyocyte Isolation System Reference: LK003300 Kit for performing five separate tissue dissociations each containing up to twelve hearts. Contains single use vials of purified collagenase and trypsin, CMF-HBSS, Leibovitz L-15 media and Falcon cell strainers along with a detailed protocol. The kit is use-tested by Worthington to assure performance.