Papain Dissociation System Reference: LK003150 Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
Papain Dissociation System Reference: LK003153 Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's balanced salt solution (EBSS), and an inhibitor vial for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Use-tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3-0.4 cm3 each.
Papain Dissociation System, Without EBSS Reference: LK003160 Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).
Papain Dissociation System, Without EBSS Reference: LK003163 Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).
DNase Vial (D2) Reference: LK003170 A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
DNase Vial (D2) Reference: LK003172 A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
PDS Kit, Papain Vial Reference: LK003176 A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1 mM L-cysteine with 0.5 mM EDTA.
PDS Kit, Papain Vial Reference: LK003178 A component of the Papain Dissociation System, for use in the tissue dissociation method of Huettner, J., and Baughman, R., J. Neuroscience, 6, 3044 (1986). Contains papain, L-cysteine, and EDTA. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of EBSS or equivalent yields a solution at 20 units of papain per ml in 1 mM L-cysteine with 0.5 mM EDTA.
PDS Kit, Inhibitor Vial Reference: LK003182 Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and lyophilized in autoclaved vials to contain 10 mg/ml each upon reconstitution with 32 ml of EBSS.
PDS Kit, EBSS Vial Reference: LK003188 Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.
Cell Isolation Optimizing System Reference: LK003200 A complete method development kit containing an assortment of enzymes most frequently used in tissue dissociation and cell isolation procedures. Includes instructions, references and strategies for the handling, use and optimization of enzymatic cell isolation methods to achieve maximum yield of viable cells. Kit includes 500 mg of each of four types of collagenase, 500 mg trypsin, 50 ku hyaluronidase, 100 mg elastase, 100 mg papain, 25 mg DNase I, 10 mg neutral protease (Dispase®) and 100 mg trypsin inhibitor.
HBSS Solution Reference: LK003210 Sterile calcium and magnesium free Hank's balanced salt solution (CMFHBSS), pH 7.4, as supplied in the NCIS kit; 1 x 500 ml.