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Reference: CL-2107-50

This cell lysate is suitable as positive control for Western Blotting, to confirm TrkB phosphorylation at amino acid S478 (rat/mouse) or S479 (human), respectively, using TrkB (pS478/479) rabbit antibody R-1718-50. It is particular useful for complex Western Blotting samples to identify TrkB (pS478/479) immunoreactive bands. This lysate has been prepared by triggering TrkB phosphorylation in retinoic acid-treated mouse NSC34 cells with mature BDNF, and subsequent processing with RIPA buffer. The cell lysate is provided lyophilised for extended stability.

Reference: 293PVS001-1

293 cells were treated with Pervanadate at 37°C for 10 min. Cells were solubilzed at 4 x 10⁶ cells/100µl in lysis buffer.

Reference: H1993S001-1

H1993 cells were cultured at 37°C to reach 85% confluence. Cells were solubilzed at 4 x 10⁶ cells/100µl in lysis buffer.

Reference: HBMPS001-1

HeLa cells were treated with BMP2. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.

Reference: HELAIGFS001-1

Hela cells were treated with IGF. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.

Reference: HELANOCS001-1

HeLa cells were treated with Nocodazole at 37°C for 20 hours. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.

Reference: HELAS001-1

HeLa cells were treated with PMA at 37°C for 20 min. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.

Reference: HELAUVS001-1

HeLa cells were treated with UV. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.