TRAIL-R1 to -R4 Flow Cytometry Pack Reference: AG-44B-0004 TRAIL-R1, -R2, -R3 and -R4 are receptors for the cytotoxic ligand TRAIL. When signaling through TRAIL-R1 and -R2, the adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. They promote the activation of NF-kappaB. TRAIL-R3 lacks a cytoplasmic death domain, and TRAIL-R4 contains a truncated death domain, and hence are not capable of inducing apoptosis. They protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.
Genotyping Kit Reference: 2GTK-100 DNA Extraction: A fast, easy, single tube lysis, producing quality, PCR ready, DNA in 15 minutes. Amplification: Using Microzone’s 2x MegaMix-GT PCR Mastermix that includes an enhanced PCR reaction buffer, dNTPs and an inert blue loading dye to enable direct gel loading post PCR. Suitable for all templates including GC rich. Enough DNA for numerous typings.
Genotyping Kit Reference: 2GTK-250 DNA Extraction: A fast, easy, single tube lysis, producing quality, PCR ready, DNA in 15 minutes. Amplification: Using Microzone’s 2x MegaMix-GT PCR Mastermix that includes an enhanced PCR reaction buffer, dNTPs and an inert blue loading dye to enable direct gel loading post PCR. Suitable for all templates including GC rich. Enough DNA for numerous typings.
Genotyping Kit Reference: 2GTK-500 DNA Extraction: A fast, easy, single tube lysis, producing quality, PCR ready, DNA in 15 minutes. Amplification: Using Microzone’s 2x MegaMix-GT PCR Mastermix that includes an enhanced PCR reaction buffer, dNTPs and an inert blue loading dye to enable direct gel loading post PCR. Suitable for all templates including GC rich. Enough DNA for numerous typings.
EnhancedFasL, Soluble (human) (rec.) Pack Reference: AG-44B-0001 FasL, Soluble (human) (rec.) induces apoptosis in a concentration range of <1ng/ml in the presence of 0.1 to 1µg/ml of TNF Ligands Enhancer (Prod. No. AG-35B-0001). (Optimal conditions must be determined individually for each cell line tested)
EnhancedTRAIL, Soluble (human) (rec.) Pack Reference: AG-44B-0002 Induces apoptosis in a concentration range of 5-50ng/ml in the presence of 1µg/ml of TNF Ligands Enhancer (Prod. No. AG-35B-0001).
Ultra Filter Unit (MW cut off 10kDa) Reference: JAI-OPF-010E Ultra filter unit for serum pretreatment. Cut-off MW is 10kDa for serum 8-OHdG.
Cytoplasmic and Nuclear Protein Extraction Kit Reference: AR0106 Cytoplasmic and Nuclear Extraction Kit, For protein extraction in WB
Cell Counting Kit-8 (CCK-8) Reference: AR1160 Cell Counting Kit-8 (CCK-8), For quantitation of viable cell number in proliferation and cytotoxicity assays