TRAIL-R1 to -R4 Flow Cytometry Pack Reference: AG-44B-0004 TRAIL-R1, -R2, -R3 and -R4 are receptors for the cytotoxic ligand TRAIL. When signaling through TRAIL-R1 and -R2, the adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. They promote the activation of NF-kappaB. TRAIL-R3 lacks a cytoplasmic death domain, and TRAIL-R4 contains a truncated death domain, and hence are not capable of inducing apoptosis. They protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.
TrkB (pS478) Positive Control Cell Lysate (Mouse NSC-34)-50 µg Reference: CL-2107-50 This cell lysate is suitable as positive control for Western Blotting, to confirm TrkB phosphorylation at amino acid S478 (rat/mouse) or S479 (human), respectively, using TrkB (pS478/479) rabbit antibody R-1718-50. It is particular useful for complex Western Blotting samples to identify TrkB (pS478/479) immunoreactive bands. This lysate has been prepared by triggering TrkB phosphorylation in retinoic acid-treated mouse NSC34 cells with mature BDNF, and subsequent processing with RIPA buffer. The cell lysate is provided lyophilised for extended stability.
293 Cell Lysate, Pervanadate Treated Reference: 293PVS001-1 293 cells were treated with Pervanadate at 37°C for 10 min. Cells were solubilzed at 4 x 10⁶ cells/100µl in lysis buffer.
COS Cell Lysate, Forskolin Treated Reference: CFSKS001-1 COS cells were treated with Forskolin. Solubilize cells at 4 x 10⁶ cells/100µl in cell lysis buffer.
H1993 Cell Lysate, Forskolin Treated Reference: H1993S001-1 H1993 cells were cultured at 37°C to reach 85% confluence. Cells were solubilzed at 4 x 10⁶ cells/100µl in lysis buffer.
HeLa Cell Lysate, BMP2 Treated Reference: HBMPS001-1 HeLa cells were treated with BMP2. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.
HeLa Cell Lysate, Anisomycin Treated Reference: HelaA001-1 HeLa cells were treated with Anisomycin. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.
HeLa Cell Lysate, Calyculin A Treated Reference: HELACALYA001-1 HeLa cells were treated with Calyculin A. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.
Hela Cell Lysate, IGF Treated Reference: HELAIGFS001-1 Hela cells were treated with IGF. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.
HeLa Cell Lysate, Nocodazole Treated Reference: HELANOCS001-1 HeLa cells were treated with Nocodazole at 37°C for 20 hours. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.
HeLa Cell Lysate, PMA Treated Reference: HELAS001-1 HeLa cells were treated with PMA at 37°C for 20 min. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.
HeLa Cell Lysate, Calyculin A and TNF-a Treated Reference: HELATCS001-1 HeLa cells were treated with Calyculin A and TNF-a. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.