TrkB (pS478) Positive Control Cell Lysate (Mouse NSC-34)-50 µg Reference: CL-2107-50 This cell lysate is suitable as positive control for Western Blotting, to confirm TrkB phosphorylation at amino acid S478 (rat/mouse) or S479 (human), respectively, using TrkB (pS478/479) rabbit antibody R-1718-50. It is particular useful for complex Western Blotting samples to identify TrkB (pS478/479) immunoreactive bands. This lysate has been prepared by triggering TrkB phosphorylation in retinoic acid-treated mouse NSC34 cells with mature BDNF, and subsequent processing with RIPA buffer. The cell lysate is provided lyophilised for extended stability.
Caspase-8, 10 Activity Fluorometric Assay Kit Reference: RP10238K Caspase-8, 10 Activity Fluorometric Assay Kit can be used for assaying caspase-8, 10, 3, 7 activities in cell/tissue extracts in a 96-well plate format. Each of the supplied fluorogenic substrate and the pan caspase inhibitor Z-VAD-FMK are sufficient for 250 x 100 μl reactions. The supplied TRAIL-treated cell lysate is used as a positive control, sufficient for 5 X 100 μl reactions.
Cytoplasmic and Nuclear Protein Extraction Kit Reference: AR0106 Cytoplasmic and Nuclear Extraction Kit, For protein extraction in WB
Cell Counting Kit-8 (CCK-8) Reference: AR1160 Cell Counting Kit-8 (CCK-8), For quantitation of viable cell number in proliferation and cytotoxicity assays