Lipid Droplets Fluorescence Assay Kit Reference: 500001-480 Cayman’s Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets.(17124) Furthermore, changes in lipid droplet biogenesis in various cell types in response to different manipulations can be both qualified and quantified by fluorescence microscopy, flow cytometry, or fluorescent plate readers. Simultaneous visualization of lipid droplets and associated proteins is also possible when used with antibodies conjugated to compatible fluorophores. Oleic acid, which is commonly used to induce lipid droplet formation in cultured cells, is included as a positive control. This kit provides sufficient reagents to effectively treat/stain 480 individual wells of cells when utilized in a 96-well plate format.
Phagocytosis Assay Kit (IgG FITC) Reference: 500290-1 Cayman’s Phagocytosis Assay Kit (IgG FITC) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-FITC Complex for up to 750 samples.
COX (ovine) Inhibitor Screening Assay Kit Reference: 560101-96 The COX (ovine) Inhibitor Screening Assay directly measures PGF2α produced in the cyclooxygenase reaction. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major prostaglandin compounds. Thus, this Cayman COX Assay is more accurate and reliable than an assay based on peroxidase inhibition. The Cayman COX (ovine) Inhibitor Screening Assay includes both ovine COX-1 and COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.