SREBP-2 Transcription Factor Assay Kit Reference: 10007819-96 SREBP-2 is a transcription factor that performs a critical role in the transcriptional regulation of genes involved in cholesterol synthesis and uptake including HMG-CoA synthase, HMG-CoA reductase, and the LDL receptor.
NF-κB (p65) Transcription Factor Assay Kit Reference: 10007889-96 The NF-κB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-κB. Acting as dimers, these transcription factors bind to κB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-κB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting human NF-κB (p65) DNA binding activity in nuclear extracts and whole cell lysates. It will not cross-react with NF-κB (p50).
Thioredoxin Reductase Colorimetric Assay Kit Reference: 10007892-96 Cayman’s TrxR Assay provides a convenient method for quantifying mammalian TrxR activity from tissue homogenates and cell lysates in a colorimetric 96-well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.