FSP1 Fluorescent Inhibitor Screening Assay Kit Reference: 701900-96 Cayman's FSP1 Fluorescent Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human FSP1, a negative regulator of the ferroptosis pathway. The assay measures the reduction of resazurin by FSP1 into resorufin, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 540 and 590 nm, respectively. The potent and reversible FSP1 inhibitor iFSP1 is included as a positive control and inhibits FSP1 with an IC50 value of 267 ± 47 nM.
Glutathione Assay Kit Reference: 703002-480 Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative protocol. The GSH Assay can be used for plasma, tissue samples, and cultured cells with minimal sample processing.