Calcium Assay Kit Reference: 701220-2 Cayman's Calcium Assay provides a quick, reliable method for determining total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates. The assay utilizes an optimized variant of the well-established o-Cresolphthalein-calcium reaction in which a vivid purple complex that absorbs between 560 and 590 nm is formed in the presence of calcium.
COX (human) Inhibitor Screening Assay Kit Reference: 701230-96 The COX (human) Inhibitor Screening Assay Kit directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using a broadly specific antiserum that binds to all the major prostaglandin compounds. This assay includes human recombinant COX-1 and COX-2, allowing the user to screen specific inhibitors. It is an excellent tool for general inhibitor screening or for eliminating false positive leads generated by less specific methods.
TMRE Mitochondrial Membrane Potential Assay Kit Reference: 701310-500 Cayman’s TMRE Mitochondrial Membrane Potential Assay Kit utilizes tetramethylrhodamine ethyl ester (TMRE), a cell permeable, cationic dye which accumulates in the mitochondrial matrix based on mitochondrial membrane potential (ΔψM). This assay, which is suitable for high-throughput screening, includes the mitochondrial uncoupler FCCP as a positive control for membrane depolarization.