SARS-CoV-2 Inhibitor Control Reference: 402055-1 SARS-CoV-2 Inhibitor Control has been tested and formulated to work exclusively with Cayman’s SARS-CoV-2 Spike-ACE2 Interaction Inhibitor Screening Assay Kit
D-Lactate Assay Kit Reference: 700520-96 D(−)-Lactate is a stereoisomer of lactate and is present in blood at only 1-5% the concentration of L(+)-lactate. Exogenous sources of D-lactate include fermented foods such as yogurt, sauerkraut, and pickles. D-lactate is normally produced in the gastrointestinal tract, mainly by lactobacilli and bifidobacteria or is produced in the cytosol of cells by the glyoxylase pathway. Cayman’s D-Lactate Assay provides a fluorescence-based method for detecting D-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, D-lactate dehydrogenase catalyzes the oxidation of D-lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Spike Inhibitor Screening Reagent Reference: 402056-1 Spike Inhibitor Screening Reagent has been tested and formulated to work exclusively with Cayman’s SARS-CoV-2 Spike-ACE2 Interaction Inhibitor Screening Assay Kit
Hemoglobin Colorimetric Assay Kit Reference: 700540-2 Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Colorimetric Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.
ACE2 Inhibitor Screening Reagent Reference: 402057-1 Spike Inhibitor Screening Reagent has been tested and formulated to work exclusively with Cayman’s SARS-CoV-2 Spike-ACE2 Interaction Inhibitor Screening Assay Kit
PAD4 Inhibitor Screening Assay Kit (Ammonia) Reference: 700560-96 Protein Arginine Deiminase 4 (PAD4) is a guanidino-modifying enzyme that functions as a transcriptional coregulator catalyzing the conversion of specific arginine residues to citrulline. Substrates for PAD4 include histones H2A, H3, and H4. PAD4 autocitrullinates itself at several sites, inhibiting its enzymatic activity. PAD4 activity is increased in rheumatoid arthritis, producing an abundance of citrulline-containing proteins that generate an immune response resulting in production of autoantibodies that ultimately attack the host tissues. PAD4 has also been implicated in several other diseases including multiple sclerosis, Alzheimer’s disease, glaucoma, and cancer. Cayman’s PAD4 Inhibitor Screening Assay (Ammonia) provides a convenient, fluorescence-based method for screening human PAD4 inhibitors.