Thiol Detection Assay Kit Reference: 700340-96 The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol Detection provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilizes a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380-390 nm and emission wavelengths between 510-520 nm.
Ascorbate Assay Kit Reference: 700420-96 Ascorbate (L-Ascorbic acid or Vitamin C) is a six-carbon lactone that is synthesized from glucose in the liver of most mammalian species, but not by humans who must obtain ascorbate through dietary consumption. In humans, ascorbate serves as an antioxidant and acts as an electron donor for various enzymes. Cayman’s Ascorbate Assay provides a reproducible, sensitive fluorescence-based tool for quantifying ascorbate from plasma, serum, urine, and fruit juices.
Creatinine (serum) Colorimetric Assay Kit Reference: 700460-2 Creatinine is commonly measured in urine and is a key benchmark for the normalization of a variety of urinary biomarkers. Serum creatinine levels, however, are a useful indicator of renal function and abnormal levels have been implicated in diabetes, cardiovascular, and circulatory diseases. Cayman’s Creatinine (serum) Colorimetric Assay is designed to measure creatinine levels in plasma and serum. The assay relies on the Jaffe' reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate. The rate of color development is directly proportional to the concentration of creatinine in the sample and is measured at an absorbance between 490-500 nm. The kinetic nature of the assay eliminates interference from extraneous serum contaminants, such as lipids and bilirubin.