Multi-Parameter Apoptosis Assay Kit Reference: 601280-100 Cayman's Multi-Parameter Apoptosis Assay Kit is a simple way to probe for multiple different apoptotic readouts at the same time. This kit employs FITC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells, TMRE as a probe for Δψm, RedDotTM2 as an indicator of plasma membrane permeability/cell viability, and Hoechst Dye to demonstrate nuclear morphology. The kit allows phenotypic characterization of multiple different cell death parameters at the single-cell level. The reagents provided in the kit are sufficient to run 100 samples.
ROS Detection Cell-Based Assay Kit (DHE) Reference: 601290-96 Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of ROS to form ethidium (E+) (ex 480 nm/em 576 nm).(32281,32282) Given their narrow spectral range, distinguishing between the two species using filter-based optical systems is often difficult and can lead to misreporting of the species of ROS being generated. This assay kit uses DHE as a fluorescent probe for the detection of ROS generation. Antimycin A, an inhibitor of complex III of the mitochondrial electron transport chain, is included as a positive control for ROS generation. N-acetyl Cysteine is included as an antioxidant control.
Early Apoptosis Detection Assay Kit Reference: 601360-100 Cayman's Early Apoptosis Detection Assay Kit employs FITC-conjugated annexin V as a probe for phosphatidylserine (PS) on the outer membrane of apoptotic cells, TMRE as a probe for ΔΨm, DAPI as an indicator of membrane permeability/cell viability, and TO-PRO®-3 to indicate the selective permeability of pannexin channels. The Early Apoptosis Detection Assay Kit allows phenotypic characterization of multiple different cell death parameters at the single-cell level.