Category: Reagents

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  • Brand: Abclonal
  • Brand: Biosensis
Reference: B-100-PS

Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis.

Phospho-Sure RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.

Reference: B-200-PS

Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phospho-proteins to phosphatases and proteases thereby resulting in uncontrolled dephosphorylation and proteolysis.

Phospho-Sure RTD Neuronal extraction buffer is optimized for the extraction of phosphorylated proteins from neuronal and other soft tissues types. The buffer extracts the phosphoproteins in a native state without the use of harsh detergents or oxidizers, and it is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent based extraction buffers.

Reference: BL-001-1250

BL-001-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce the sIgA cross-reactivity present in certain substrates such as milk.

Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-001-1250:

BEK-2211, Mature BDNF; Application: human milk

One vial of BL-001-1250 contains 1250 μg of proprietary immunoglobulins, which is sufficient as sample diluent additive for one 96-well plate.

Other ELISA assays may also benefit from addition of blocker BL-001-1250, but require optimization of working concentration and assay validation for accurate results.

Reference: BL-002-1250

BL-002-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents.

One vial of BL-002-1250 contains 1250 μg IgG which is sufficient as sample diluent additive for one 96-well plate if used at 50 μg/mL. Optimal blocker concentration needs to be validated by the end-user for accurate results.

Reference: BL-003-1000

BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents.

Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-003-1000:

BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma
BEK-2218, Human NT4/5; Application: Citrate-Plasma

One vial of BL-003-1000 contains 1000 μg IgG which is sufficient as sample diluent additive for one 96-well plate.

Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.

Reference: BL-004-500

BL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents.

Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-004-500:

BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-Plasma
BEK-2221, Human NT3; Application: Plasma (Citrate and EDTA)
BEK-2237, Human proBDNF; Application: Serum, EDTA-Plasma

One vial of BL-004-500 contains 500 μg IgG which is sufficient as sample diluent additive for one 96-well plate.

Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.