Recombinant human SUMO-2 protein Reference: RP10115LQ SUMO (small Ub-related modifier) is a Ub-like protein. Three types of SUMO are most commonly studied, SUMO 1, SUMO 2, and SUMO 3. SUMO 2 and SUMO 3 are almost identical isoforms and thus share many functions. Like Ub, SUMO can be conjugated to its target proteins as a polymeric chain. However, SUMO 1 forms chains inefficiently as compared to SUMO 2 and SUMO 3. SUMO is conjugated to target proteins by the E1 (SAE1/SAE2), E2 (Ube2I or Ubc9), E3 (RanBP2/Nup358, amongst others). Protein sumoylation is involved in many cellular processes including gene transcription.
Recombinant human SUMO-3 protein Reference: RP10117LQ SUMO (small Ub-related modifier) is a Ub-like protein. Three types of SUMO are most commonly studied, SUMO 1, SUMO 2, and SUMO 3. SUMO 2 and SUMO 3 are almost identical isoforms and thus share many functions. Like Ub, SUMO can be conjugated to its target proteins as a polymeric chain. However, SUMO 1 forms chains inefficiently as compared to SUMO 2 and SUMO 3. SUMO is conjugated to target proteins by the E1 (SAE1/SAE2), E2 (Ube2I or Ubc9), E3 (RanBP2/Nup358, amongst others). Protein sumoylation is involved in many cellular processes including gene transcription.
Recombinant human TAK1-binding protein 2/TAB-2(627-693) (NZF) protein Reference: RP10118LQ GST was fused on the N-terminus of the lysine 63 polyubiquitin chain binding domain of TAB2 encompassing amino acids 627-693. This fusion protein can be used for in vitro GST pulldown assays and for enrichment of cellular proteins conjugated with lysine 63 polyubiquitin chains in whole cell or tissue lysates. GST-TAB2 (NZF) can be precipitated using glutathione resin. After washing, GST-TAB2 (NZF) and its bound proteins can be eluted by a buffer containing 10 mM glutathione.
Recombinant human TNF-alpha protein Reference: RP10119LQ TNFalpha (Tumor necrosis factor alpha) is produced by immune and epithelial cells. It plays important roles in regulating inflammation, apoptosis, and immune response. It exerts its function mainly by binding of two cell surface receptors TNFR1 and TNFR2. Treating cells with TNFalpha activates the NFkappaB, MAPK, and apoptotic signaling pathways. The recombinant GST-TNFalpha protein consists of a N-terminal GT tag with the C-terminal extracellular domain (amino acids 77-233) of full length TNFalpha. It can be used for pulldown assays. It binds TNFR1 and TNFR2 to induce downstream signaling pathways, but often requires higher concentrations than TNFalpha (catalog # U1120).
Recombinant human Ubiquitin-conjugating enzyme E2 K/UBE2K protein Reference: RP10120LQ Ube2K (or E2-25K) is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. It contains a C-terminal UBA domain and an E2 catalytic domain. Ube2K contains a 47 amino acid residue tail that contributes greatly to its specific characteristics. Ube2K is one of the E2s that are capable of forming K48-linked polyubiquitin chains.
Recombinant human Ubiquitin-protein ligase E3A/UBE3A protein Reference: RP10121LQ UbE3A (also called E6AP) is a 100 kDa HECT (homologous to E6AP carboxyl terminus) domain ubiquitin ligase containing two E2 binding sites for UbcH7 and UbcH8. UbE3A was found to bind to the human papillomavirus (HPV) oncoprotein E6 and mediate ubiquitination of p53. A frameshift mutation in the UbE3A gene leads to loss of function and causes Angelman Syndrome.
Recombinant human Ubiquitin protein Reference: RP10122LQ Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. This product can be used for pulldwon assay to determine ubiquitin-binding proteins.
Recombinant human Ubiquitin carboxyl-terminal hydrolase 21/USP21 protein Reference: RP10129LQ USP21(195-565) contains the catalytic domain of USP21 and it is capable of cleavage of different types of polyubiquitin chains. USP21 can deubiquitinate histone H2A, a specific tag for epigenetic transcriptional repression. It can also cleave Nedd8 conjugates.
Recombinant human Ubiquitin carboxyl-terminal hydrolase... Reference: RP10130LQ USP30(57-517) contains the catalytic domain of USP30 with the N-terminal membrane bound domain being deleted. USP30 deubiquitinates substrates of Parkin including MIRO1, TOMM20, MFN1, and MFN2 on mitochondrial membranes and functions to suppress mitophagy. It prefers cleavage of K6- and K11-linked polyubiquitin chains.
Recombinant human Ubiquitin carboxyl-terminal hydrolase 4/USP4 protein Reference: RP10131LQ Usp4 is a member of the ubiquitin-specific protease family of deubiquitinating enzyme. A number of substrates of Usp4 have been identified, including TRIM21, TRAF2, TRAF6, RIG-1, RIP1, PDK1, TAK1 and etc. Usp4-mediated deubiquitination was found to regulate several signaling pathways, including the AKT signaling, RIG-1-mediated antiviral response, TNFα-induced signaling, and the Wnt signaling.
Recombinant human Sentrin-specific protease 2(catalytic domain)/SENP2 protein Reference: RP10132LQ USP9X belongs to the ubiquitin-specific protease (USP) family. USP9X has been found to deubiqitinate more than a dozen of substrates including mcl1, survivin, SMAD4, SMN and Itch. USP9X is located on the X chromosome, but it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Also, Usp9X is unregulated in various cancers. The catalytic domain contains the region from amino acid 1531 to amino acid 1972.
Recombinant human ZnF-UBP/USP5 protein Reference: RP10133LQ Usp5 (IsoT) is a deubiquitinase enzyme that hydrolyzes free polyubiquitin chains. The Usp5 contains two zinc finger domains (ZnF-UBP). This product contains the second ZnF-UBP domain (amino acid 163 – 291). The ZnF-UBP domains bind free ubiquitin chains’ diglycine carboxyl tail, which defines its specificity in binding of free polyubiquitin chains.