Protein Aggregation Fluorometric Assay Kit

Cayman’s Protein Aggregation Assay Kit provides a fast and convenient method to determine the level of protein aggregation in a purified antibody solution or other protein solutions after generating an appropriate standard curve using the protein of interest. The assay uses a Protein Aggregation Dye that binds to exposed hydrophobic regions, resulting in an increase in fluorescence, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 490 and 550 nm, respectively. Aggregated and unaggregated antibody solutions are included as positive and negative controls, respectively. The limit of the detection of the the assay is less than 1% aggregation.