Category: Molecular Biology Reagents

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  • Categories: Nucleotides
  • Categories: Retrotranscriptases
  • Brand: HighQu
Reference: NUM0101

highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature storage or long PCR of more than 30 kb targets, as well as long amplifications exceeding 40 cycles.

Reference: RTM0301

The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.

Reference: NUM0201

highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature storage or long PCR of more than 30 kb targets, as well as long amplifications exceeding 40 cycles.

Reference: RTM0305

The HighScriber™ Reverse Transcriptase Mix is a premium tool for the high efficiency reverse transcription of up to 12-15 kb long cDNA. Mix includes HighScriber™ Reverse Transcriptase and Ribonuclease Inhibitor for save, robust cDNA synthesis and ease of use. HighScriber™ Reverse Transcriptase allows for high detection sensitivity from 1 pg of total RNA. The wide reaction temperature range (38°C - 55°C) ensures efficient cDNA synthesis from complex or GC rich templates. The enzyme uses ssRNA or ssDNA as a template, possesses no detectable Ribonuclease H activity specific to RNA in RNA-DNA hybrids. A highly reduced Ribonuclease H activity allows for transcription of full lengths long transcripts. HighScriber™ Reverse Transcriptase can be used for RACE as it has terminal transferase activity - adds cytosines to 3’ ends of cDNA. The Ribonuclease inhibitor premixed with the RT ensures RNA protection from ribonuclease degradation. Supplied 5X ALLin™ HighScriber Buffer includes everything you need for the cDNA synthesis reaction. To minimize pipetting steps it contains MgCl2, dNTPs, enhancers, stabilizers. The only things to add is the template RNA and primer.

Reference: NUS0101

highQu dNTP sets and mixes meet all highest industry standards and allow for unrivaled performance of your PCR and other DNA synthesis, labeling or sequencing reactions. Produced under the stringent quality monitoring conditions, they guaranty reproducible results. More than 99% HPLC purity eliminates inhibitions of PCR and allows for increased yields with higher dNTP concentrations. Exceptional stability of dNTPs allows for short term ambient temperature shipments, short term room temperature storage or long PCR of more than 30 kb targets, as well as long amplifications exceeding 40 cycles.

Reference: RTK0101

The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets. The highly active and thermostable HighScriber™ Reverse Transcriptase blended with RNase Inhibitor allows for an efficient cDNA synthesis and reaction safety. The wide reaction temperature range (38°C - 55°C) ensures efficient transcription from GC rich templates. The 5X qScriber™ Reaction Mix includes optimal concentrations of magnesium and dNTPs and a combination of anchored oligo (dT) and random hexamers for unbiased representation of mRNA ends. The kit is an optimal choice for generating high quality cDNA from viral RNA, miRNA or other targets for qPCR or for PCR. For the maximum convenience the Kit includes even the PCR Water to set up the reaction, so the only thing you need to take care of is the high quality RNA template.

Reference: RTK0104

The qScriber™ cDNA Synthesis Kit is a highly efficient and simple-to-use system for cDNA synthesis eliminating the need for tedious reaction optimization. The qScriber™ Enzyme Blend ensures high sensitivity detection from low copy number targets. The highly active and thermostable HighScriber™ Reverse Transcriptase blended with RNase Inhibitor allows for an efficient cDNA synthesis and reaction safety. The wide reaction temperature range (38°C - 55°C) ensures efficient transcription from GC rich templates. The 5X qScriber™ Reaction Mix includes optimal concentrations of magnesium and dNTPs and a combination of anchored oligo (dT) and random hexamers for unbiased representation of mRNA ends. The kit is an optimal choice for generating high quality cDNA from viral RNA, miRNA or other targets for qPCR or for PCR. For the maximum convenience the Kit includes even the PCR Water to set up the reaction, so the only thing you need to take care of is the high quality RNA template.