Category: Molecular Biology Reagents

Reference: R401-01

RNA isolater Total RNA Extraction Reagent, based on guanidine isothiocyanate and phenol, has a strong lysis ability, which can lyse cells and tissue samples in a short time to ensure the integrity of RNA. This product is widely used for culturing cells, animal tissues, microorganisms, and plant tissues with less secondary metabolism, such as seedlings and young leaves. The sample can be fully lysed in RNA isolater. After centrifugation with chloroform, the solution will form a supernatant layer, an intermediate layer and an organic layer (bright red lower layer). RNA is distributed in the upper aqueous phase. After the supernatant layer is collected, the Total RNA can be recovered by propanol precipitation.Application: Based on guanidine isothiocyanate and phenol, strong cracking ability; The optimized lysate formula has a wide range of sample adaptability.

Reference: R701-01

RNA-easy Isolation Reagent is widely used to extract Total RNA and Small RNA from samples of various animal tissues, plant materials, cultured cells and microorganisms. Compared with the traditional Trizol extraction method, this product is simple to use, does not require the use of chloroform for stratification, and the whole process can be performed at room temperature; in addition, this product effectively inhibits the activity of RNase (RNase) while simultaneously removing protein and DNA. , Polysaccharides and other impurities precipitate to the bottom of the tube to achieve single-phase extraction and effectively ensure the integrity and purity of RNA. The entire extraction process can be completed within 50 minutes using this product. The extracted RNA can be directly used in various molecular biology such as cDNA cloning, qRT-PCR detection, mRNA purification, in vitro translation, Northern blotting hybridization, high-throughput sequencing, etc. experiment.Application: Broad platform compatibility: The addition of universal ROX to miRNA Universal SYBR® qPCR Master Mix enables mix to be compatible with qPCR instruments on different platforms without the need to adjust ROX concentration.

Reference: RC101-01

This kit can quickly extract total RNA from animal cells or tissues. The kit is based on silica gel column purification technology. There is no need to use toxic phenol/chloroform extraction during the extraction process. The whole process only takes 30 minutes. The gDNA-Filter Columns in the kit can effectively separate the supernatant and adsorb and remove gDNA; RNAPure Columns can efficiently bind RNA, with optimized Buffer solution, so that the total RNA obtained is of high purity, free of protein and other impurities, and can be used Used in various downstream experiments such as RT-PCR, Real-Time PCR, chip analysis, etc.Application: High purity; Few genome residues; Simple and fast Strong versatility

Reference: RC112-01

The FastPure Cell/Tissue Total RNA Isolation Kit V2 provides a rapid method for extracting total  RNA from animal tissues and cells. The kit is based on silica gel membrane purification technology  and does not require β-mercaptoethanol, phenol/chloroform, or any other toxic reagent during  the extraction process. It takes only 6 min to extract high-quality RNA. The kit contains FastPure  gDNA-Filter Columns III which can effectively remove impurities and gDNA. FastPure RNA  Columns III can efficiently bind RNA with an optimized buffer to obtain high-purity total RNA. The  isolated RNA has little gDNA residues and no proteins or other impurities contamination. It can  be used for RT-PCR, Real-Time PCR, Microarrarys and other downstream experiments.

Reference: RC201

MiPure Cell / Tissue miRNA Kit is suitable for extracting small RNA (miRNA, <200 nt) from less than 5 x 106 eukaryotic cells or less than 100 mg animal tissues. The resulting miRNA has high purity without large RNA or genome DNA contamination can be directly used for chip analysis, Northern hybridization, RT-PCR and miRNA library construction. This kit combines high-efficiency RNA Isolater extraction reagents and silica gel column purification technology to complete the extraction of miRNA within 1 hour; it can also be used to separate and extract large molecular RNA (>200 nt) or total RNA (including miRNA) to satisfy users Different needs.Application: The miRNA extraction efficiency is high, and the starting amount is wide; Larger RNA and genomic DNA have fewer residues; Easy to operate, strong compatibility

Reference: RC401

This kit can quickly extract the total RNA of multiple samples from plant tissues, especially plant tissues rich in polysaccharides and polyphenols (such as cotton leaves, pine needles, bananas, potato tubers, grapes, rose flowers, buckwheat, etc.). The kit is based on silica gel column purification technology. There is no need to use toxic phenol-chloroform extraction during the extraction process. The whole process only takes 30 minutes. The FastPure gDNA-Filter Column II in the kit can effectively filter impurities and specifically adsorb and remove genomic DNA. FastPure RNA Column Ⅳ can efficiently bind RNA, and is equipped with optimized Buffer solution, so that the total RNA obtained is of high purity, free of protein and other impurities, and can be used for RT-PCR, Real-time PCR, chip analysis, Northern Blot And other downstream experiments.

Reference: R411-01

VeZol Reagent is intended for the isolation of high-quality total RNA from cultured cells, animal tissues, and plant tissues. VeZol Reagent is a monophasic solution of phenol and guanidine isothiocyanate supplemented with optimized proprietary components for more effective sample disruption and RNase inhibition. After rapid sample lysis with VeZol Reagent and chloroform extraction, the lysate separates into an upper aqueous phase (containing RNA), an interphase, and a lower red organic phase, and total RNA can be obtained in high yield and purity by isopropanol precipitation and ethanol wash. The resulting total RNA can be used directly for RT-PCR, RT-qPCR, Northern blot, Dot blot, in vitro translation, high-throughput sequencing, and other molecular biology experiments.

Features

Ultra classical organic extraction

Super lysis capability

RNA extraction from a wide variety of samples are available, such as cells, tissues, simple plants and blood.

Reference: R411-02

VeZol Reagent is intended for the isolation of high-quality total RNA from cultured cells, animal tissues, and plant tissues. VeZol Reagent is a monophasic solution of phenol and guanidine isothiocyanate supplemented with optimized proprietary components for more effective sample disruption and RNase inhibition. After rapid sample lysis with VeZol Reagent and chloroform extraction, the lysate separates into an upper aqueous phase (containing RNA), an interphase, and a lower red organic phase, and total RNA can be obtained in high yield and purity by isopropanol precipitation and ethanol wash. The resulting total RNA can be used directly for RT-PCR, RT-qPCR, Northern blot, Dot blot, in vitro translation, high-throughput sequencing, and other molecular biology experiments.

Features

Ultra classical organic extraction

Super lysis capability

RNA extraction from a wide variety of samples are available, such as cells, tissues, simple plants and blood.

Reference: R711-01

FreeZol Reagent is widely applicable to the extraction of total RNA from cultured cells, animal  tissues, and simple plant tissues. Compared with the conventional Trizol method, this product  features a simple procedure that can be performed at room temperature with no need to use  chloroform for phase separation. In addition, this product ensures the integrity and purity of  the extracted RNA by precipitating proteins, DNA, polysaccharides and other impurities in the  organic phase while retaining RNA in the upper aqueous phase. The whole procedure can be  completed in 1 h. The obtained total RNA can be used directly for RT-PCR, qRT-PCR, Northern blot, Dot blot, in vitro translation, NGS, and other molecular biology experiments.

Features  

Safe and low in toxicity: No need for toxic and harmful reagents such as chloroform and β-mercaptoethanol

Ease to use: RNA extraction in a monophasic system, no phase separation, all operations at room temperature

High RNA quality: High purity and integrity of RNA at a yield comparable to the traditional Trizol method

Reference: RC411-01

FastPure Universal Plant Total RNA Isolation Kit can be used to quickly extract total RNA from plant tissues. The kit includes two solution systems designed for simple RNA extraction from a variety of plant tissues (wheat, rice, corn, Arabidopsis thaliana, tobacco, rape, etc.), polysaccharide and polyphenol rich plant tissues (cotton leaf, soybean leaf, pine needle, ginkgo leaf, fig leaf, gardenia leaf, wheat seed, corn seed, red bean seed, potato, sweet potato, soybean seed, sesame seed, peanut seeds, rapeseed, etc.), fruit pulp (watermelon, apple, peach, pear, banana, mango, etc.), and fungi (shiitake, button mushroom, oyster mushroom, Neurospora crassa, etc.). The kit is based on silica column purification technology and does not involve toxic reagents (e.g., phenol or chloroform and β-mercaptoethanol) or time-consuming alcohol precipitation. The whole extraction process takes only 11 min. FastPure gDNA-Filter Columns III in the kit effectively remove impurities and gDNA. FastPure RNA Columns V efficiently bind to RNA and, paired with optimized buffers, yield high-purity total RNA with minimal gDNA residue and no protein or other impurities that can be used for a range of downstream experiments, including RT-PCR, real-time PCR, RNA library preparation, microarray analysis, Northern blot, dot blot, and molecular cloning.

Reference: P611-01

HiScript® II One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrate HiScript® II Reverse Transcriptase and the superior performance of Champagne TaqTM plus DNA Polymerase designed for long fragment amplification, and cooperate with the optimized buffer system HiScript® II One Step RT-PCR Kit to amplify fragments up to 10 kb or more. The kit is provided in a convenient Master Mix format. 2 × One Step Mix contains optimized buffer system and dNTP; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.Application: Reverse transcription and PCR are performed in the same tube The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments over 10 kb

Reference: P612-01

HiScript® II One Step RT-PCR Kit (Dye Plus) is specially designed for end-point PCR detection using RNA as a template (such as RNA virus). PCR products can be directly loaded for electrophoresis, which greatly saves time and labor. Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript® II Reverse Transcriptase and Champagne TaqTM plus DNA Polymerase, with an optimized buffer system, the detection sensitivity of HiScript® II One Step RT-PCR Kit (Dye Plus) can reach 1 pg total RNA and can be amplified Fragments up to 10 kb. The kit is provided in a convenient Master Mix format. 2 × One Step Mix (Dye Plus) contains optimized buffer system, dNTP and loading dye; One Step Enzyme Mix contains optimized ratio of HiScript® II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.Application: Reverse transcription and PCR are performed in the same tube; The detection sensitivity can reach 0.1 pg total RNA; Amplify long fragments up to 10 kb or more; PCR products can be directly loaded for electrophoresis