Category: Molecular Biology Reagents

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  • Categories: DNA Extraction
  • Brand: Minerva
  • Brand: Vazyme
Reference: R701-02

RNA-easy Isolation Reagent is widely used to extract Total RNA and Small RNA from samples of various animal tissues, plant materials, cultured cells and microorganisms. Compared with the traditional Trizol extraction method, this product is simple to use, does not require the use of chloroform for stratification, and the whole process can be performed at room temperature; in addition, this product effectively inhibits the activity of RNase (RNase) while simultaneously removing protein and DNA. , Polysaccharides and other impurities precipitate to the bottom of the tube to achieve single-phase extraction and effectively ensure the integrity and purity of RNA. The entire extraction process can be completed within 50 minutes using this product. The extracted RNA can be directly used in various molecular biology such as cDNA cloning, qRT-PCR detection, mRNA purification, in vitro translation, Northern blotting hybridization, high-throughput sequencing, etc. experiment.Application: Easy to operate: single-phase extraction, no need for multi-phase stratification; the whole process can be operated at room temperature; Wide compatibility: It is widely applicable to various animal, plant and microbial samples, and it can easily cope with various cultured cells; High RNA quality: RNA has high purity, good integrity, and yield comparable to traditional Trizol extraction; Safety and health: No need to use toxic and harmful reagents such as chloroform.

Reference: DC111-01

This kit is mainly suitable for quickly and easily extracting genomic DNA from 0.1-1 ml fresh and frozen anticoagulated whole blood samples. The kit uses silica gel membrane purification technology, and no toxic reagents such as phenol and chloroform are required during the extraction process. There is no need for time-consuming alcohol precipitation, and the maximum removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as enzyme digestion, PCR, Southern blotting, etc. Application: High yield, excellent purity, good integrity; wide range of sample application; wide range of volume compatibility

Reference: DC111-02

This kit is mainly suitable for quickly and easily extracting genomic DNA from 0.1-1 ml fresh and frozen anticoagulated whole blood samples. The kit uses silica gel membrane purification technology, and no toxic reagents such as phenol and chloroform are required during the extraction process. There is no need for time-consuming alcohol precipitation, and the maximum removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as enzyme digestion, PCR, Southern blotting, etc. Application: High yield, excellent purity, good integrity; wide range of sample application; wide range of volume compatibility

Reference: DC102-01

This product is suitable for the extraction of genomic DNA from 1-20 mg animal tissues and cultured cell samples less than 5 × 106. The kit is based on silica gel column purification technology. There is no need to use phenol/chloroform organic solvent extraction and alcohol precipitation during the extraction process. It only takes 30-60 minutes to obtain high-purity and high-yield genomic DNA, which can be directly used in the enzyme digestion reaction. , PCR, qPCR, library construction and Southern hybridization experiments.Application: Simple and fast; Wide adaptability; High purity; High security

Reference: DC103-01

This kit is suitable for extracting genomic DNA from various sources of bacteria (Gram-negative bacteria, Gram-positive bacteria). The kit uses silica gel membrane purification technology. There is no need to use toxic reagents such as phenol and chloroform during the extraction process, and no time-consuming alcohol precipitation, and to maximize the removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as restriction digestion, PCR, and Southern hybridization.Application: Simple and fast; Wide range of applications; High yield and good integrity; High purity

Reference: DC104-01

This kit is suitable for extracting genomic DNA from fresh and dried common plant samples or polysaccharide polyphenol plant samples. The kit uses silica gel membrane purification technology and a new and unique solution system. There is no need to use toxic reagents such as phenol, chloroform, or time-consuming alcohol precipitation during the extraction process, which can maximize the removal of impurity proteins and other organic compounds in plant cells. The extracted genomic DNA has high purity and stable quality, and can be used in molecular biology experiments such as PCR, enzyme digestion and hybridization.Application: Simple and fast; Wide range of applications; High yield and good integrity; High purity

Reference: DC112-01

This kit is mainly suitable for extracting genomic DNA from a variety of fresh or frozen anticoagulant blood, cells, animal tissues and bacterial samples. The kit uses silica gel membrane purification technology, no need to use toxic reagents such as phenol, chloroform, and time-consuming alcohol precipitation during the extraction process, and to maximize the removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as restriction digestion, PCR, and Southern hybridization.Application: High quality and good integrity: The extracted DNA has the characteristics of high yield, good purity, and complete fragments. Wide range of samples: suitable for direct extraction of DNA from a variety of fresh or frozen anticoagulated blood, cells, animal tissues and bacterial samples. Simple and fast: Animal tissue provides a 30min quick extraction plan.

Reference: DC112-02

This kit is mainly suitable for extracting genomic DNA from a variety of fresh or frozen anticoagulant blood, cells, animal tissues and bacterial samples. The kit uses silica gel membrane purification technology, no need to use toxic reagents such as phenol, chloroform, and time-consuming alcohol precipitation during the extraction process, and to maximize the removal of RNA, protein, lipids and other inhibitory impurities. The extracted genomic DNA has high purity and stable quality, and can be used for downstream experiments such as restriction digestion, PCR, and Southern hybridization.Application: High quality and good integrity: The extracted DNA has the characteristics of high yield, good purity, and complete fragments. Wide range of samples: suitable for direct extraction of DNA from a variety of fresh or frozen anticoagulated blood, cells, animal tissues and bacterial samples. Simple and fast: Animal tissue provides a 30min quick extraction plan.

Reference: DC202-01

This kit is suitable for extracting 150-300 ml of overnight cultured bacteria, using an improved SDS-alkaline lysis method to lyse the bacteria, the crude extract is selectively combined with a unique endotoxin scavenger and centrifuged to remove endotoxins, and then The silicon matrix membrane in the centrifugal adsorption column selectively binds the plasmid DNA in the solution under high salt and low pH conditions, and then removes impurities and other bacterial components through the rinsing solution, and finally elution with low salt and high pH The buffer eluted purified plasmid DNA from the silicon matrix membrane. The silicon matrix membrane in the centrifugal adsorption column all adopts imported special adsorption membrane, the adsorption amount difference between the column and the column is very small, and the repeatability is good. There is no need to use toxic phenol, chloroform and other reagents, nor ethanol precipitation. It can quickly extract 0.2-1.5 mg of pure high-copy plasmid DNA with an extraction rate of 80-90%. The unique process formula removes endotoxin, the content of endotoxin is very low (< 0.1 EU/μg DNA), and the cell transfection effect is excellent. It can also be directly used in various molecular biology experiments such as enzyme digestion, PCR, in vitro transcription, transformation, sequencing, and so on.Application: High Yield; Compatible with the extraction of plasmids of different lengths; The content of endotoxin is extremely low, and subsequent transfection level experiments can be done; Good stability

Reference: DE103

Lysozyme (lysozyme) is an alkaline enzyme that can hydrolyze mucopolysaccharides in bacteria. It mainly destroys the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall. The cell wall insoluble mucopolysaccharide is decomposed into soluble glycopeptides, causing the cell wall to rupture and the bacteria to dissolve. Mainly used for the digestion of the cell wall of gram-positive bacteria during the preparation of DNA and RNA.Application: Equipped with optimized high-efficiency buffer; Simple, fast and efficient, suitable for the cell wall digestion of Gram-positive bacteria

Reference: DC301-01

This kit uses an optimized buffer system and silica gel column purification technology, which can recover 70 bp-20 kb DNA fragments from various concentrations of TAE or TBE agarose gels, and transfer them to the DNA adsorption column under high salt conditions. Direct centrifugation can specifically adsorb DNA and remove other impurities. In addition, this kit can directly purify DNA fragments from PCR products, enzymatic reaction systems or crude DNA products obtained by various other methods, while removing impurities such as proteins, other organic compounds, inorganic salt ions, and oligonucleotide primers. . It can ensure that the purification work is completed in 10-15 min. The purified DNA can be directly used for molecular biology research such as ligation, transformation, restriction enzyme digestion, in vitro transcription, PCR, sequencing, and microinjection.Application: Simple and fast; One box for dual use; High recovery efficiency; Wide range of recovered DNA fragments

Reference: DC105-01

FastPure FFPE DNA Isolation Kit is intended for isolating genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue, overcoming the inhibitory effect caused by formalin crosslinking. The deparaffinization solution in this kit is safer than xylene. The DNA-specific spin column and the unique buffer system also ensure the high quality of obtained DNA. The whole extraction process takes only 20 min (digestion time not counted). The extracted genomic DNA is of good integrity, high purity, and stable quality and can be used for a wide range of downstream applications, including PCR, qPCR, and library preparation.