ClonExpress II One Step Cloning Kit Reference: C112-01 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same sequence as the two ends of the linearized vector (15 bp-20 bp). This PCR product and the linearized vector are in a certain ratio After mixing, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning. As a new generation recombinant cloning kit, ClonExpress® II has a unique non-ligase dependent system, which greatly reduces the background of vector self-ligation, and the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps. Application: Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%
E.Z.N.A.® Blood RNA Kit Reference: R6814-01 The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 mL of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenized with a homogenizer spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as hemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC-treated water.
E.Z.N.A.® Plant DNA DS Mini Kit Reference: D2411-00 The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
5x BaseQ Reference: 3BAQ28 A straightforward 5 x dilution buffer designed for sequencing reactions to be used as an extremely powerful replacement for ABI dilution buffers.
SwabUpTM Lab Monitoring Reference: 181-0010 Kits for the regular monitoring of lab work area and detection of target and amplicon DNA contaminations.
ClonExpress II One Step Cloning Kit Reference: C112-02 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same sequence as the two ends of the linearized vector (15 bp-20 bp). This PCR product and the linearized vector are in a certain ratio After mixing, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning.As a new generation recombinant cloning kit, ClonExpress® II has a unique non-ligase dependent system, which greatly reduces the background of vector self-ligation, and the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps. Application: Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%
E.Z.N.A.® Plant RNA Kit Reference: R6827-02 E.Z.N.A.® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogenizer column for filtration and homogenization of viscous plant cell lysate by centrifugation in combination with the HiBind RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, Northern blot analysis, differential display, and poly A+ RNA selection.
E.Z.N.A.® Plant DNA DS Mini Kit Reference: D2411-01 The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
Just Water Reference: 5JWA-50 Just Water is available for all of your essential requirements at a price that will get you coming back. We don’t compromise on quality. Microzone has been offering Just Water for many years to all of our customers. RNAase, DNase free.
SwabUpTM Lab Monitoring Reference: 181-0050 Kits for the regular monitoring of lab work area and detection of target and amplicon DNA contaminations.
ClonExpress MultiS One Step Cloning Kit Reference: C113-01 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize the vector, and perform forward/reverse PCR on the insert. The 5'end of the primer is introduced into the end sequence of the linearized vector, so that the 5'and 3'ends of the PCR product have the same sequence (15 bp-20 bp) as the two ends of the linearized vector. After the PCR product of the sequence and the linearized vector are mixed in a certain proportion, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning.As a new generation of recombination cloning kit, ClonExpress® MultiS is specifically optimized for multi-fragment recombination reactions, which can realize sequential splicing cloning of up to five inserts at a time. The unique non-ligase-dependent system of the kit greatly reduces the background of vector self-ligation, and does not need to consider the enzyme cleavage site carried by the insert itself; the highly optimized reaction buffer and the enhanced recombinase Exnase MultiS can significantly improve The recombination efficiency of the clone and the tolerance to impurities make it possible to directly use the linearized vector and insert for recombination cloning without purification, which greatly simplifies the experimental steps. Application: Multi-fragment splicing cloning can be performed at almost any position of any vector; No need to consider the restriction site carried by the insert itself; Sequential splicing of two to five inserts can be realized at one time; Linearized cloning vector and PCR products can be cloned directly without purification
E.Z.N.A.® Tissue Direct PCR Kit Reference: TQ2310-00 The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.