miRNA 1st Strand cDNA Synthesis Kit (by tailing A) Reference: MR201-01 miRNA 1st strand cDNA Synthesis Kit (by tailing A) is a kit for microRNA (miRNA) reverse transcription (RT) by tailing A. Poly A polymerase (PAP) adds a Poly(A) tail to the 3' end of miRNA or total RNA, and then universal reverse transcription primers initiate RT reaction with reverse transcriptase. HiScript miRNA Enzyme Mix contains bioengineered reverse transcriptase and PAP. Combined with the optimized buffer, the reverse transcription of non-miRNAs is effectively inhibited, achieving highly specific qPCR results. 2 × miRNA RT Mix and HiScript miRNA Enzyme Mix contains all components required for miRNA tailing and reverse transcription. Two reactions can be performed simultaneously in one tube.
E.Z.N.A.® Blood RNA Kit Reference: R6814-01 The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 mL of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenized with a homogenizer spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as hemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC-treated water.
E.Z.N.A.® Plant DNA DS Mini Kit Reference: D2411-00 The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
5x BaseQ Reference: 3BAQ28 A straightforward 5 x dilution buffer designed for sequencing reactions to be used as an extremely powerful replacement for ABI dilution buffers.
100CFU™ Mycoplasma Sensitivity Standards for Mycoplasma fermentans Reference: 103-6003 Contains 3 vials each with 100 CFU of inactivated mycoplasma particles and 2 negative controls. For extensive validation of detection limit and robustness.
E.Z.N.A.® Plant RNA Kit Reference: R6827-02 E.Z.N.A.® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogenizer column for filtration and homogenization of viscous plant cell lysate by centrifugation in combination with the HiBind RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, Northern blot analysis, differential display, and poly A+ RNA selection.
E.Z.N.A.® Plant DNA DS Mini Kit Reference: D2411-01 The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
Just Water Reference: 5JWA-50 Just Water is available for all of your essential requirements at a price that will get you coming back. We don’t compromise on quality. Microzone has been offering Just Water for many years to all of our customers. RNAase, DNase free.
100CFU™ Mycoplasma Sensitivity Standards for Mycoplasma hyorhinis Reference: 103-7003 Contains 3 vials each with 100 CFU of inactivated mycoplasma particles and 2 negative controls. For extensive validation of detection limit and robustness.
E.Z.N.A.® Tissue Direct PCR Kit Reference: TQ2310-00 The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.