E.Z.N.A.® Tissue Direct PCR Kit Reference: TQ2310-00 The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.
E.Z.N.A.® HP Plant DNA Mini Kit Reference: D2485-00 The E.Z.N.A.® HP Plant DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples rich in lipids, polyphenols, polysaccharides, or those with lower DNA content. Up to 100 mg of wet sample or 30 mg dry sample can be processed in less than 60 minutes. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek's HiBind® matrix. Samples are homogenized and lysed in a high salt buffer containing CTAB and extracted with chloroform to remove polysaccharides and other components that interfere with many DNA isolation and downstream applications. After adjusting the binding conditions, DNA is further purified using HiBind DNA spin columns. Proteins and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification and hybridization techniques.
ALLin™ Mega HiFi DNA Polymerase Reference: HLE0305 Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors. For maximum convenience, the ALLin™ Mega HiFi Mastermix, 2X (HLM0201) and ALLin™ Mega HiFi Red Mastermix, 2X (HLM0301) are available.
E.Z.N.A.® Tissue Direct PCR Kit Reference: TQ2310-01 The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.
E.Z.N.A.® HP Plant DNA Mini Kit Reference: D2485-02 The E.Z.N.A.® HP Plant DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples rich in lipids, polyphenols, polysaccharides, or those with lower DNA content. Up to 100 mg of wet sample or 30 mg dry sample can be processed in less than 60 minutes. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek's HiBind® matrix. Samples are homogenized and lysed in a high salt buffer containing CTAB and extracted with chloroform to remove polysaccharides and other components that interfere with many DNA isolation and downstream applications. After adjusting the binding conditions, DNA is further purified using HiBind DNA spin columns. Proteins and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification and hybridization techniques.
ALLin™ Mega HiFi Mastermix Reference: HLM0201 Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors. For maximum convenience, the ALLin™ Mega HiFi Mastermix, 2X (HLM0201) and ALLin™ Mega HiFi Red Mastermix, 2X (HLM0301) are available.
E.Z.N.A.® Tissue Direct PCR Kit Reference: TQ2310-02 The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.
E.Z.N.A.® Blood DNA Maxi Kit Reference: D2492-00 The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large-scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 20 mL whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.
ALLin™ Mega HiFi Mastermix Reference: HLM0205 Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors. For maximum convenience, the ALLin™ Mega HiFi Mastermix, 2X (HLM0201) and ALLin™ Mega HiFi Red Mastermix, 2X (HLM0301) are available.