ClonExpress MultiS One Step Cloning Kit Reference: C113-01 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize the vector, and perform forward/reverse PCR on the insert. The 5'end of the primer is introduced into the end sequence of the linearized vector, so that the 5'and 3'ends of the PCR product have the same sequence (15 bp-20 bp) as the two ends of the linearized vector. After the PCR product of the sequence and the linearized vector are mixed in a certain proportion, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning.As a new generation of recombination cloning kit, ClonExpress® MultiS is specifically optimized for multi-fragment recombination reactions, which can realize sequential splicing cloning of up to five inserts at a time. The unique non-ligase-dependent system of the kit greatly reduces the background of vector self-ligation, and does not need to consider the enzyme cleavage site carried by the insert itself; the highly optimized reaction buffer and the enhanced recombinase Exnase MultiS can significantly improve The recombination efficiency of the clone and the tolerance to impurities make it possible to directly use the linearized vector and insert for recombination cloning without purification, which greatly simplifies the experimental steps. Application: Multi-fragment splicing cloning can be performed at almost any position of any vector; No need to consider the restriction site carried by the insert itself; Sequential splicing of two to five inserts can be realized at one time; Linearized cloning vector and PCR products can be cloned directly without purification
E.Z.N.A.® Tissue Direct PCR Kit Reference: TQ2310-00 The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.
E.Z.N.A.® HP Plant DNA Mini Kit Reference: D2485-00 The E.Z.N.A.® HP Plant DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples rich in lipids, polyphenols, polysaccharides, or those with lower DNA content. Up to 100 mg of wet sample or 30 mg dry sample can be processed in less than 60 minutes. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek's HiBind® matrix. Samples are homogenized and lysed in a high salt buffer containing CTAB and extracted with chloroform to remove polysaccharides and other components that interfere with many DNA isolation and downstream applications. After adjusting the binding conditions, DNA is further purified using HiBind DNA spin columns. Proteins and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification and hybridization techniques.
ClonExpress MultiS One Step Cloning Kit Reference: C113-02 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize the vector, and perform forward/reverse PCR on the insert. The 5'end of the primer is introduced into the end sequence of the linearized vector, so that the 5'and 3'ends of the PCR product have the same sequence (15 bp-20 bp) as the two ends of the linearized vector. After the PCR product of the sequence and the linearized vector are mixed in a certain proportion, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning. As a new generation of recombination cloning kit, ClonExpress® MultiS is specifically optimized for multi-fragment recombination reactions, which can realize sequential splicing cloning of up to five inserts at a time. The unique non-ligase-dependent system of the kit greatly reduces the background of vector self-ligation, and does not need to consider the enzyme cleavage site carried by the insert itself; the highly optimized reaction buffer and the enhanced recombinase Exnase MultiS can significantly improve The recombination efficiency of the clone and the tolerance to impurities make it possible to directly use the linearized vector and insert for recombination cloning without purification, which greatly simplifies the experimental steps. Application: Multi-fragment splicing cloning can be performed at almost any position of any vector; No need to consider the restriction site carried by the insert itself; Sequential splicing of two to five inserts can be realized at one time; Linearized cloning vector and PCR products can be cloned directly without purification
E.Z.N.A.® Tissue Direct PCR Kit Reference: TQ2310-01 The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.
E.Z.N.A.® HP Plant DNA Mini Kit Reference: D2485-02 The E.Z.N.A.® HP Plant DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples rich in lipids, polyphenols, polysaccharides, or those with lower DNA content. Up to 100 mg of wet sample or 30 mg dry sample can be processed in less than 60 minutes. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek's HiBind® matrix. Samples are homogenized and lysed in a high salt buffer containing CTAB and extracted with chloroform to remove polysaccharides and other components that interfere with many DNA isolation and downstream applications. After adjusting the binding conditions, DNA is further purified using HiBind DNA spin columns. Proteins and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification and hybridization techniques.
ClonExpress Ultra One Step Cloning Kit Reference: C115-01 ClonExpress® Ultra One Step Cloning Kit is a new generation of recombinant cloning kit, which can quickly and efficiently clone 1-5 fragments to any position of any vector. The highly optimized 2×ClonExpress Mix, 50℃ reaction 5-15 Min completes the reorganization, and the operation is simple and quick.Application: Fast: 5-15min rapid reorganization; Simple: One-tube Mix, easy to operate; High efficiency: Highly efficient cloning of 50 bp～10 kb fragments, with a positive rate of >95%; Compatible: single fragment, multi-fragment, entry clone three in one; Zero background: provide zero background, ampicillin/canal double resistance carrier
E.Z.N.A.® Tissue Direct PCR Kit Reference: TQ2310-02 The Tissue Direct PCR Kit incorporates a novel buffer system that effectively lyses tissue and neutralizes inhibitors. Simply take small pieces of tissue (5-10 mg) and incubate with T1 Buffer at room temperature or 56°C for 10 minutes to lyse tissue and release DNA. After a 3 minute incubation at 95°C, the sample is ready for PCR amplification.
E.Z.N.A.® Blood DNA Maxi Kit Reference: D2492-00 The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large-scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 20 mL whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.