Fluorescent LAMP/RT-LAMP Kit Reference: RP711-02 Fluorescent LAMP/RT-LAMP Kit is designed to provide a simple one-step solution for loop-mediated isothermal amplification of DNA or RNA targets. The 2 × Fluorescent LAMP/RT-LAMP Master Mix in this kit contains optimized buffer solution, dNTP, Bst II Pro DNA polymerase and RTv reverse transcriptase for fast and efficient LAMP/RT-LAMP detection. Bst II Pro DNA Polymerase and RTv Reverse Transcriptase use the updated hot start technology, which has excellent specificity and supports the preparation of reaction system at room temperature. This kit provides fluorescent dyes to realize real-time fluorescence detection of LAMP/RT-LAMP.
E-Z 96 Plant DNA DS Kit Reference: D1411-01 The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethylammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB, binding conditions are adjusted, and DNA is purified using an E-Z 96® DNA Plate. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
5x BaseQ Reference: 3BAQ-1 A straightforward 5 x dilution buffer designed for sequencing reactions to be used as an extremely powerful replacement for ABI dilution buffers.
ALLin™ HiFi DNA Polymerase Reference: HLE0201 highQu ALLin™ HiFi DNA Polymerase is the outperforming high-fidelity enzyme derived from Pfu polymerase by introducing several point mutations. The robust engineered enzyme in combination with the optimized ALLin™ buffer provides higher fidelity (50X higher than Taq DNA Polymerase), better performance, increased success in demanding applications like amplification of complex or longer templates, crude samples and fast cycling. ALLin™ HiFi DNA Polymerase produces blunt-ended products suitable for ligating into blunt vectors.
miRNA 1st Strand cDNA Synthesis Kit (by tailing A) Reference: MR201-01 miRNA 1st strand cDNA Synthesis Kit (by tailing A) is a kit for microRNA (miRNA) reverse transcription (RT) by tailing A. Poly A polymerase (PAP) adds a Poly(A) tail to the 3' end of miRNA or total RNA, and then universal reverse transcription primers initiate RT reaction with reverse transcriptase. HiScript miRNA Enzyme Mix contains bioengineered reverse transcriptase and PAP. Combined with the optimized buffer, the reverse transcription of non-miRNAs is effectively inhibited, achieving highly specific qPCR results. 2 × miRNA RT Mix and HiScript miRNA Enzyme Mix contains all components required for miRNA tailing and reverse transcription. Two reactions can be performed simultaneously in one tube.
E.Z.N.A.® Plant DNA DS Mini Kit Reference: D2411-00 The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
5x BaseQ Reference: 3BAQ28 A straightforward 5 x dilution buffer designed for sequencing reactions to be used as an extremely powerful replacement for ABI dilution buffers.
ALLin™ HiFi DNA Polymerase Reference: HLE0205 highQu ALLin™ HiFi DNA Polymerase is the outperforming high-fidelity enzyme derived from Pfu polymerase by introducing several point mutations. The robust engineered enzyme in combination with the optimized ALLin™ buffer provides higher fidelity (50X higher than Taq DNA Polymerase), better performance, increased success in demanding applications like amplification of complex or longer templates, crude samples and fast cycling. ALLin™ HiFi DNA Polymerase produces blunt-ended products suitable for ligating into blunt vectors.
E.Z.N.A.® Plant DNA DS Mini Kit Reference: D2411-01 The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
Just Water Reference: 5JWA-50 Just Water is available for all of your essential requirements at a price that will get you coming back. We don’t compromise on quality. Microzone has been offering Just Water for many years to all of our customers. RNAase, DNase free.
ALLin™ Mega HiFi DNA Polymerase Reference: HLE0301 Derived from our HiFi polymerase, the highQu ALLin™ Mega HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. The ALLin™ Mega HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HiFi DNA Polymerase is an excellent choice for longer and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors. For maximum convenience, the ALLin™ Mega HiFi Mastermix, 2X (HLM0201) and ALLin™ Mega HiFi Red Mastermix, 2X (HLM0301) are available.