FastPure Plant Total RNA Isolation Kit (Polysaccharides / Polyphenolics-Rich) Reference: RC401 This kit can quickly extract the total RNA of multiple samples from plant tissues, especially plant tissues rich in polysaccharides and polyphenols (such as cotton leaves, pine needles, bananas, potato tubers, grapes, rose flowers, buckwheat, etc.). The kit is based on silica gel column purification technology. There is no need to use toxic phenol-chloroform extraction during the extraction process. The whole process only takes 30 minutes. The FastPure gDNA-Filter Column II in the kit can effectively filter impurities and specifically adsorb and remove genomic DNA. FastPure RNA Column Ⅳ can efficiently bind RNA, and is equipped with optimized Buffer solution, so that the total RNA obtained is of high purity, free of protein and other impurities, and can be used for RT-PCR, Real-time PCR, chip analysis, Northern Blot And other downstream experiments.
VeZol Reagent Reference: R411-01 VeZol Reagent is intended for the isolation of high-quality total RNA from cultured cells, animal tissues, and plant tissues. VeZol Reagent is a monophasic solution of phenol and guanidine isothiocyanate supplemented with optimized proprietary components for more effective sample disruption and RNase inhibition. After rapid sample lysis with VeZol Reagent and chloroform extraction, the lysate separates into an upper aqueous phase (containing RNA), an interphase, and a lower red organic phase, and total RNA can be obtained in high yield and purity by isopropanol precipitation and ethanol wash. The resulting total RNA can be used directly for RT-PCR, RT-qPCR, Northern blot, Dot blot, in vitro translation, high-throughput sequencing, and other molecular biology experiments.FeaturesUltra classical organic extractionSuper lysis capabilityRNA extraction from a wide variety of samples are available, such as cells, tissues, simple plants and blood.
VeZol Reagent Reference: R411-02 VeZol Reagent is intended for the isolation of high-quality total RNA from cultured cells, animal tissues, and plant tissues. VeZol Reagent is a monophasic solution of phenol and guanidine isothiocyanate supplemented with optimized proprietary components for more effective sample disruption and RNase inhibition. After rapid sample lysis with VeZol Reagent and chloroform extraction, the lysate separates into an upper aqueous phase (containing RNA), an interphase, and a lower red organic phase, and total RNA can be obtained in high yield and purity by isopropanol precipitation and ethanol wash. The resulting total RNA can be used directly for RT-PCR, RT-qPCR, Northern blot, Dot blot, in vitro translation, high-throughput sequencing, and other molecular biology experiments.FeaturesUltra classical organic extractionSuper lysis capabilityRNA extraction from a wide variety of samples are available, such as cells, tissues, simple plants and blood.
FreeZol Reagent Reference: R711-01 FreeZol Reagent is widely applicable to the extraction of total RNA from cultured cells, animal tissues, and simple plant tissues. Compared with the conventional Trizol method, this product features a simple procedure that can be performed at room temperature with no need to use chloroform for phase separation. In addition, this product ensures the integrity and purity of the extracted RNA by precipitating proteins, DNA, polysaccharides and other impurities in the organic phase while retaining RNA in the upper aqueous phase. The whole procedure can be completed in 1 h. The obtained total RNA can be used directly for RT-PCR, qRT-PCR, Northern blot, Dot blot, in vitro translation, NGS, and other molecular biology experiments.Features Safe and low in toxicity: No need for toxic and harmful reagents such as chloroform and β-mercaptoethanolEase to use: RNA extraction in a monophasic system, no phase separation, all operations at room temperatureHigh RNA quality: High purity and integrity of RNA at a yield comparable to the traditional Trizol method
FastPure Universal Plant Total RNA Isolation Kit Reference: RC411-01 FastPure Universal Plant Total RNA Isolation Kit can be used to quickly extract total RNA from plant tissues. The kit includes two solution systems designed for simple RNA extraction from a variety of plant tissues (wheat, rice, corn, Arabidopsis thaliana, tobacco, rape, etc.), polysaccharide and polyphenol rich plant tissues (cotton leaf, soybean leaf, pine needle, ginkgo leaf, fig leaf, gardenia leaf, wheat seed, corn seed, red bean seed, potato, sweet potato, soybean seed, sesame seed, peanut seeds, rapeseed, etc.), fruit pulp (watermelon, apple, peach, pear, banana, mango, etc.), and fungi (shiitake, button mushroom, oyster mushroom, Neurospora crassa, etc.). The kit is based on silica column purification technology and does not involve toxic reagents (e.g., phenol or chloroform and β-mercaptoethanol) or time-consuming alcohol precipitation. The whole extraction process takes only 11 min. FastPure gDNA-Filter Columns III in the kit effectively remove impurities and gDNA. FastPure RNA Columns V efficiently bind to RNA and, paired with optimized buffers, yield high-purity total RNA with minimal gDNA residue and no protein or other impurities that can be used for a range of downstream experiments, including RT-PCR, real-time PCR, RNA library preparation, microarray analysis, Northern blot, dot blot, and molecular cloning.