Fluorescent LAMP/RT-LAMP Kit Reference: RP711-02 Fluorescent LAMP/RT-LAMP Kit is designed to provide a simple one-step solution for loop-mediated isothermal amplification of DNA or RNA targets. The 2 × Fluorescent LAMP/RT-LAMP Master Mix in this kit contains optimized buffer solution, dNTP, Bst II Pro DNA polymerase and RTv reverse transcriptase for fast and efficient LAMP/RT-LAMP detection. Bst II Pro DNA Polymerase and RTv Reverse Transcriptase use the updated hot start technology, which has excellent specificity and supports the preparation of reaction system at room temperature. This kit provides fluorescent dyes to realize real-time fluorescence detection of LAMP/RT-LAMP.
E-Z 96 Plant DNA DS Kit Reference: D1411-01 The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethylammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB, binding conditions are adjusted, and DNA is purified using an E-Z 96® DNA Plate. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
5x BaseQ Reference: 3BAQ-1 A straightforward 5 x dilution buffer designed for sequencing reactions to be used as an extremely powerful replacement for ABI dilution buffers.
miRNA 1st Strand cDNA Synthesis Kit (by tailing A) Reference: MR201-01 miRNA 1st strand cDNA Synthesis Kit (by tailing A) is a kit for microRNA (miRNA) reverse transcription (RT) by tailing A. Poly A polymerase (PAP) adds a Poly(A) tail to the 3' end of miRNA or total RNA, and then universal reverse transcription primers initiate RT reaction with reverse transcriptase. HiScript miRNA Enzyme Mix contains bioengineered reverse transcriptase and PAP. Combined with the optimized buffer, the reverse transcription of non-miRNAs is effectively inhibited, achieving highly specific qPCR results. 2 × miRNA RT Mix and HiScript miRNA Enzyme Mix contains all components required for miRNA tailing and reverse transcription. Two reactions can be performed simultaneously in one tube.
ClonExpress II One Step Cloning Kit Reference: C112-01 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same sequence as the two ends of the linearized vector (15 bp-20 bp). This PCR product and the linearized vector are in a certain ratio After mixing, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning. As a new generation recombinant cloning kit, ClonExpress® II has a unique non-ligase dependent system, which greatly reduces the background of vector self-ligation, and the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps. Application: Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%
E.Z.N.A.® Plant DNA DS Mini Kit Reference: D2411-00 The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
5x BaseQ Reference: 3BAQ28 A straightforward 5 x dilution buffer designed for sequencing reactions to be used as an extremely powerful replacement for ABI dilution buffers.
ClonExpress II One Step Cloning Kit Reference: C112-02 ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the vector and place the insert 5'in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end, so that the 5'and 3'ends of the PCR product have the same sequence as the two ends of the linearized vector (15 bp-20 bp). This PCR product and the linearized vector are in a certain ratio After mixing, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning.As a new generation recombinant cloning kit, ClonExpress® II has a unique non-ligase dependent system, which greatly reduces the background of vector self-ligation, and the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification, greatly simplifying The experimental steps. Application: Simple, fast, efficient, suitable for any carrier; No need to consider the restriction site carried by the insert; Efficient cloning of 50 bp-10 kb DNA fragments; Linearized cloning vector and PCR products can be cloned directly without purification; No ligase, no self-connection, positive rate >95%
E.Z.N.A.® Plant DNA DS Mini Kit Reference: D2411-01 The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB and binding conditions are adjusted and DNA is purified using a HiBind® DNA Mini Columns. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.
Just Water Reference: 5JWA-50 Just Water is available for all of your essential requirements at a price that will get you coming back. We don’t compromise on quality. Microzone has been offering Just Water for many years to all of our customers. RNAase, DNase free.