Category: Molecular Biology Reagents

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  • Categories: Accessory PCR components
  • Categories: DNA Extraction
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  • Categories: RNA Extraction
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Reference: R6731-01

The E.Z.N.A.® DNA/RNA Isolation Kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenized in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind® RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, the maximum yield of DNA and RNA can be purified from the entire sample.

Reference: D1043-02

The E-Z 96® Cycle Pure Kit procedure allows for the parallel purification of up to 96 PCR samples from multiple amplifications. The E-Z 96® Cycle Pure Kit utilizes multiwell technology for manual or fully automated high-throughput purification. The HiBind® DNA resin selectively adsorbs DNA molecules in an optimized binding buffer (Buffer CP). After a single wash step, pure PCR products are eluted in water or Elution Buffer provided and are ready to use in subsequent applications.

Reference: 3BB-5

Another novel enhancing buffer designed for use with ABI BigDye sequencing mixes v1.0 and 3.0. Compatible with all sequencing machines (apart from MegaBASE), all you need to do is dilute your ABI BigDye to reduce product consumption and costs.

Reference: R6812-00

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Reference: D1086-01

E-Z 96® Plant DNA Kits adapted buffer system from Omega Bio-tek's SP Plant DNA system which is particularly useful to process varieties of plants, such as those with unusually high levels of phenolic compounds or polysaccharides as exemplified in cotton, pine and peanut samples. Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.

Reference: 3BB1.5-5

Another novel enhancing buffer designed for use with ABI BigDye sequencing mixes v1.0 and 3.0. Compatible with all sequencing machines (apart from MegaBASE), all you need to do is dilute your ABI BigDye to reduce product consumption and costs.

Reference: R6812-01

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Reference: D1086-02

E-Z 96® Plant DNA Kits adapted buffer system from Omega Bio-tek's SP Plant DNA system which is particularly useful to process varieties of plants, such as those with unusually high levels of phenolic compounds or polysaccharides as exemplified in cotton, pine and peanut samples. Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.