Category: Molecular Biology Reagents

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  • Categories: RNA Extraction
Reference: R6812-00

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Reference: D1086-01

E-Z 96® Plant DNA Kits adapted buffer system from Omega Bio-tek's SP Plant DNA system which is particularly useful to process varieties of plants, such as those with unusually high levels of phenolic compounds or polysaccharides as exemplified in cotton, pine and peanut samples. Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.

Reference: 3BB1.5-5

Another novel enhancing buffer designed for use with ABI BigDye sequencing mixes v1.0 and 3.0. Compatible with all sequencing machines (apart from MegaBASE), all you need to do is dilute your ABI BigDye to reduce product consumption and costs.

Reference: R6812-01

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Reference: D1086-02

E-Z 96® Plant DNA Kits adapted buffer system from Omega Bio-tek's SP Plant DNA system which is particularly useful to process varieties of plants, such as those with unusually high levels of phenolic compounds or polysaccharides as exemplified in cotton, pine and peanut samples. Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.

Reference: 3BB1.5-10

Another novel enhancing buffer designed for use with ABI BigDye sequencing mixes v1.0 and 3.0. Compatible with all sequencing machines (apart from MegaBASE), all you need to do is dilute your ABI BigDye to reduce product consumption and costs.

Reference: 57-2102

A pre-aliquoted and lyophilized reagent in high profile stripes, ready upon rehydration with buffer.

Reference: R6812-02

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Reference: D1411-00

The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. This procedure relies on the well-established properties of the cationic detergent, cetyltrimethylammonium bromide (CTAB), in conjunction with the unique binding system to increase yields and provide high-quality DNA. The system eliminates the need for chloroform extractions traditionally associated with CTAB-based lysis methods. Samples are homogenized and lysed in a high salt buffer containing CTAB, binding conditions are adjusted, and DNA is purified using an E-Z 96® DNA Plate. Salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization applications.

Reference: 3BB25

Another novel enhancing buffer designed for use with ABI BigDye sequencing mixes v1.0 and 3.0. Compatible with all sequencing machines (apart from MegaBASE), all you need to do is dilute your ABI BigDye to reduce product consumption and costs.