HiScript III One Step qRT-PCR Probe Kit Reference: Q225-EN01 HiScript III One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit can prevent the contamination of PCR product. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability. HiScript III One Step qRT-PCR Probe Kit is provided in Master Mix. The 5 × One Step Mix contains an optimized buffer and dNTP Mix, and is suitable for high-specificity detection systems based on flfluorescence labelled probes (e.g. TaqMan).
HiScript III One Step qRT-PCR Probe Kit Reference: Q225-EN02 HiScript III One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit can prevent the contamination of PCR product. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability. HiScript III One Step qRT-PCR Probe Kit is provided in Master Mix. The 5 × One Step Mix contains an optimized buffer and dNTP Mix, and is suitable for high-specificity detection systems based on flfluorescence labelled probes (e.g. TaqMan).
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) Reference: Q621-EN01 AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) is a one-tube probe-based RT-qPCR master mix suitable for single or multiple quantitative PCR detection using RNA as a template (such as RNA virus). The enzyme in this master mix uses a hot-start technology based on enzyme modification, with a new generation of high-affinity Taq enzyme antibodies, to achieve efficient blocking and precise release. Combined with one-step dedicated Reverse Transcriptase and optimized buffer, it supports pre-mixing of primers and probes in advance and the mixture can be stored for a long time at low temperature. The sample to be tested can be added directly when using, no additional tube opening/pipetting operation. This product is also compatible with fast programs, which greatly shortens the detection time.
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) Reference: Q621-EN02 AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) is a one-tube probe-based RT-qPCR master mix suitable for single or multiple quantitative PCR detection using RNA as a template (such as RNA virus). The enzyme in this master mix uses a hot-start technology based on enzyme modification, with a new generation of high-affinity Taq enzyme antibodies, to achieve efficient blocking and precise release. Combined with one-step dedicated Reverse Transcriptase and optimized buffer, it supports pre-mixing of primers and probes in advance and the mixture can be stored for a long time at low temperature. The sample to be tested can be added directly when using, no additional tube opening/pipetting operation. This product is also compatible with fast programs, which greatly shortens the detection time.
BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) Reference: QN222-01 BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) is a probe-based qPCR premix for DNA multiplex amplification, which supports premixing of primers and probes. The BioSmart DNA Polymerase is screened by Vazyme's unique BioSmart platform for enzymes with long sequence of shortest extended bases and strong 3' end mismatch recognition ability. With the high blocking rate dual-species specific antibody (binding to different antigenic epitopes) and an optimized buffer system, it effectively improves multiplex amplification sensitivity, specificity, low-copy detection rate, GC content compatibility and impurity tolerance (blood, ethanol, guanidinium salts, etc.). It supports fast program and primers and probes premixing. In addition, the dUTP/UDG anti-contamination system in 2 × All-Powerful qPCR PreMix effectively prevents aerosol contamination, ensuring accurate qPCR results.
BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) Reference: QN222-02 BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) is a probe-based qPCR premix for DNA multiplex amplification, which supports premixing of primers and probes. The BioSmart DNA Polymerase is screened by Vazyme's unique BioSmart platform for enzymes with long sequence of shortest extended bases and strong 3' end mismatch recognition ability. With the high blocking rate dual-species specific antibody (binding to different antigenic epitopes) and an optimized buffer system, it effectively improves multiplex amplification sensitivity, specificity, low-copy detection rate, GC content compatibility and impurity tolerance (blood, ethanol, guanidinium salts, etc.). It supports fast program and primers and probes premixing. In addition, the dUTP/UDG anti-contamination system in 2 × All-Powerful qPCR PreMix effectively prevents aerosol contamination, ensuring accurate qPCR results.
UniPeak U+ One Step RT-qPCR SYBR Green Kit Reference: Q226-01 UniPeak U+ One Step RT-qPCR SYBR Green Kit is a dye-based RT-qPCR reagent specifically designed for quantitative PCR detection using RNA as the template. The dye-based fluorescence quantitative detection presents two major challenges: the difficulty of detecting low-concentration samples and the issue of non-specific amplification. This product addresses these challenges with a next-generation antibody-modified Taq DNA Polymerase and a one-step, temperature-activated Reverse Transcriptase, combined with an optimized buffer system for RT-qPCR, offering enhanced sensitivity and specificity. To further ensure accurate quantification, a dUTP/UDG anti-contamination system is included, eliminating aerosol contamination at room temperature. Additionally, the 5 × gDNA Wiper effectively removes any residual genomic DNA contamination from RNA templates. The 2 × One Step SYBR Green Master Mix includes tracking dye and universal ROX Reference Dye, making it compatible with all types of qPCR instruments, requiring only the addition of primers and templates for amplification.