HiScript ® II 1st Strand cDNA Synthesis Kit（+gDNA wiper） Reference: R212-02 HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper) is a special kit for cDNA one-strand synthesis that contains a genomic DNA removal step. The HiScript® II Reverse Transcriptase based on the Kit has a significant improvement compared with the previous generation HiScript® Reverse Transcriptase. Thermal stability, template affinity and inhibitor tolerance. The gDNA wiper used in the Kit can quickly remove genomic DNA contamination at 42°C for 2 minutes, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across exons. 10 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo dT23VN included in the kit has a stronger anchoring ability for Poly A+ RNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo dT23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can synthesize full-length cDNA for cloning (up to 20 kb), but also synthesize cDNA with uniform reverse transcription efficiency for each position of qPCRApplication: Based on the highly efficient HiScript ® II Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA; A wide range of template starting amount, from 10 pg-5 μg total RNA, can amplify fragments up to 15 kb or more; Anchored Oligo(dT) 23 VN is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis; Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, etc.; gDNA wiper Mix can quickly and completely remove genome contamination
SuperKine™ West Femto Maximum Sensitivity Substrate Reference: BMU102-EN_100 mL SuperKine™ West Femto Maximum Sensitivity Substrate provides a highly sensitive color developing solution to enhance the chemical signal of immunological experiments. In order to obtain the best experimental results, you need to optimize all your experimental factors, including the number of samples, antibody concentration, the use of membranes and blocking agents.
E.Z.N.A.® PX Blood RNA Kit Reference: R1057-01 Omega Bio-tek's E.Z.N.A.® PX Blood RNA Kit is designed for isolation of total RNA from blood samples stored in special preserved reagents and Paxgene tubes. The procedure completely removes contaminants and enzyme inhibitors making RNA isolation fast, convenient, and reliable. RNA purified using the E.Z.N.A.® PX RNA Kit method is ready for applications such as RT-PCR.
CozyHi™ Prestained Protein Ladder Reference: PRL0202 highQu ready to use Prestained Protein Ladders are mixtures of highly recombinant proteins with coupled chromophores providing sharp protein bands and bright colors on denaturing polyacrylamide gels. The high ladder purity allows for exceptional stability and room short term temperature storage. Ladders are ready to be directly loaded on gels without any preparation or heating. They provide sharp bands for approximate protein sizing and allow for monitoring of electrophoresis process and Western transfer efficiency.
HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) Reference: R312-01 HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.Application: Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product; Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns
SuperKine™ Enhanced Antibody Dilution Buffer Reference: BMU103-EN_100 mL SuperKine™ Enhanced Antibody Dilution Buffer provides an enhanced reagent, which can be directly used to dilute the primary or secondary antibody in an appropriate ratio with this product.
SQ Total RNA Kit Reference: R3053-05 The E.Z.N.A.® SQ Total RNA Kit is designed for isolating total RNA from animal, tissue and cultured cells. This solution-based system can be easily scaled up and down based on the starting material. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation that are eliminated. RNA purified using the E.Z.N.A.® SQ Total RNA method can be directly used for applications such as RT-PCR, Northern blotting, and other enzymatic reactions.
CozyXL™ Prestained Protein Ladder Reference: PRL0302 highQu ready to use Prestained Protein Ladders are mixtures of highly recombinant proteins with coupled chromophores providing sharp protein bands and bright colors on denaturing polyacrylamide gels. The high ladder purity allows for exceptional stability and room short term temperature storage. Ladders are ready to be directly loaded on gels without any preparation or heating. They provide sharp bands for approximate protein sizing and allow for monitoring of electrophoresis process and Western transfer efficiency.
HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) Reference: R312-02 HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript® II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript® III Reverse Transcriptase and the most suitable for reverse transcription optimization. Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need to design primers across introns. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR quantification.Application: Based on the more efficient HiScript®III Reverse Transcriptase: reverse transcription efficiency is higher than that of the second-generation product; Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs; gDNA wiper Mix can quickly and completely remove genome contamination: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns
Universal Loading Control Antibody Cocktail Reference: KTD101-EN_1 Kit Universal Loading Control Antibody Cocktail provides a comprehensive and detailed method to quickly and conveniently detect the content of load control proteins in a variety of samples.
E.Z.N.A.® Total RNA Midi Kit Reference: R6664-00 The E.Z.N.A.® Total RNA Midi Kit is designed for RNA isolation from large amounts of sample. The E.Z.N.A.® Total RNA Midi Kit can purify total RNA from up to 1x10^8 cultured cells or 200 mg animal tissues. By using the HiBind® midi column technology, the E.Z.N.A.® Total RNA Midi Kit greatly reduces processing time when compared to traditional methods such as CsCl ultracentrifugation or phenol/chloroform protocols. Purified RNA can be used in downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.
BetterBASE Reference: 3BBR-5 Enhancing buffer designed for use with the ABI BigDye v1.1 and v3.1 sequencing mixes, for use with all sequencing machines replaces both home made and commercial dilution buffers, shown to improve results from difficult templates as well as reducing sequencing costs and is ideal for high throughput sequencing.