Category: Molecular Biology Reagents

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  • Categories: Accessory PCR components
  • Categories: DNA Extraction
  • Categories: Sample collection
Reference: D0926-01

The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.

Reference: 3BBR1.5-5

Enhancing buffer designed for use with the ABI BigDye v1.1 and v3.1 sequencing mixes, for use with all sequencing machines replaces both home made and commercial dilution buffers, shown to improve results from difficult templates as well as reducing sequencing costs and is ideal for high throughput sequencing.

Reference: ISD‐T‐250‐R

DNA collection kit for humans that can capture samples producing high yields of double stranded, long fragmented DNA from buccal cells using traditional swabs.

Reference: D0926-02

The E.Z.N.A.® Insect DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, roundworms, flatworms, invertebrates, as well as some plant tissue samples. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. The procedure relies on the well-established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind matrix. This allows salts, proteins and other contaminants to be removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.

Reference: 3BBR25

Enhancing buffer designed for use with the ABI BigDye v1.1 and v3.1 sequencing mixes, for use with all sequencing machines replaces both home made and commercial dilution buffers, shown to improve results from difficult templates as well as reducing sequencing costs and is ideal for high throughput sequencing.

Reference: ISR2‐T‐1200‐R

The iSWAB-RNA-v2 device allows for the non-invasive collection, concentration, and stabilization of intact buccal cells and/or any mammalian cells collected with a swab or cytobrush allowing for real time ambient stabilization of total RNA from the point of collection to processing. Maintaining RNA stability and protecting it from degradation is a significant challenge, while current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance. 

Reference: D1043-02

The E-Z 96® Cycle Pure Kit procedure allows for the parallel purification of up to 96 PCR samples from multiple amplifications. The E-Z 96® Cycle Pure Kit utilizes multiwell technology for manual or fully automated high-throughput purification. The HiBind® DNA resin selectively adsorbs DNA molecules in an optimized binding buffer (Buffer CP). After a single wash step, pure PCR products are eluted in water or Elution Buffer provided and are ready to use in subsequent applications.

Reference: 3BB-5

Another novel enhancing buffer designed for use with ABI BigDye sequencing mixes v1.0 and 3.0. Compatible with all sequencing machines (apart from MegaBASE), all you need to do is dilute your ABI BigDye to reduce product consumption and costs.

Reference: ISP‐T‐1200‐R

Non-invasive sample collection tube for collection and stabilization of protein and DNA in the same oral sample.  Oral sample collection has gained widespread acceptance due to its non-invasive nature and because continuous visual contact of the collection process is possible.  Current non-invasive collection products are designed for immediate detection using stick-based technology, but are limited to simple yes/no answers.  If additional data is required, a more robust collection method coupled with high-sensitivity downstream analysis is necessary.

Reference: D1086-01

E-Z 96® Plant DNA Kits adapted buffer system from Omega Bio-tek's SP Plant DNA system which is particularly useful to process varieties of plants, such as those with unusually high levels of phenolic compounds or polysaccharides as exemplified in cotton, pine and peanut samples. Up to 50 mg of wet tissue or 12 mg dry tissue can be processed in each well in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind matrix with the speed and versatility of the E-Z 96® DNA plate to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.

Reference: 3BB1.5-5

Another novel enhancing buffer designed for use with ABI BigDye sequencing mixes v1.0 and 3.0. Compatible with all sequencing machines (apart from MegaBASE), all you need to do is dilute your ABI BigDye to reduce product consumption and costs.

Reference: ISAN‐T‐250‐R

Collect DNA samples from animals with high yield with <1% bacterial contamination.  Reduce downstream failure rates for your animal genetics testing by using the iSWAB-Animal collection kit.