1Step RT qPCR Green ROX H Kit Reference: QOD0201 highQu 1Step RT qPCR master mixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR. qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the 1Step RT qPCR Green Mixes are available in two versions – with low or high ROX concentration.
1Step RT qPCR Green ROX H Kit Reference: QOD0205 highQu 1Step RT qPCR master mixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR. qPCR master mixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization. Our master mixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the 1Step RT qPCR Green Mixes are available in two versions – with low or high ROX concentration.
ALLin™ Taq DNA Polymerase Reference: PCE0101 highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the first error). ALLin™ Taq DNA Polymerase produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ Taq Mastermix, 2X is available.
ALLin™ Taq DNA Polymerase Reference: PCE0105 highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the first error). ALLin™ Taq DNA Polymerase produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ Taq Mastermix, 2X is available.
ALLin™ Red Taq Mastermix Reference: PCM0201 highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors. ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.
ALLin™ Red Taq Mastermix Reference: PCM0205 highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Red Mastermix providing the additional advantage of reduced pipetting and minimized errors. ALLin™ Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.
ALLin™ Taq Mastermix Reference: PCM0101 highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.
ALLin™ Taq Mastermix Reference: PCM0105 highQu ALLin™ Taq DNA Polymerase is the versatile engineered enzyme which in combination with the optimized ALLin™ buffer provides higher success rates in demanding PCR applications like amplification of complex templates, crude sample PCR and fast cycling. ALLin™ Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (a number of correct nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. The convenience of ALLin™ Taq DNA Polymerase is maximized by the use of 2X Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with the PCR Water, and the only thing to add is the template with primers.
Taq DNA Polymerase Reference: PCE0201 highQu Taq DNA Polymerase is the classical enzyme for routine PCR applications providing high amplification yields of 3-5 kb targets under various conditions. Taq DNA Polymerase is purified from a recombinant E. coli strain carrying the Taq DNA polymerase gene. Taq DNA polymerase is thermostable 5´ → 3´ DNA polymerase. It lacks 3´ → 5´ exonuclease (proofreading) activity and has low 5´ → 3´ exonuclease activity. Polymerase exhibits deoxynucleotidyl transferase activity resulting in A-overhang at the 3'-ends of PCR products and allowing for TA cloning. The PCR accuracy of Taq DNA Polymerase is 4.5 x 104 (a number of incorporated nucleotides before the first error occurs). The supplied reaction buffer for most flexibility in optimization, contains no dNTPs and no magnesium. The 50 mM MgCl2 solution is provided separately what allows for magnesium optimization starting from 1 mM up to 4 mM final concentration upon the need. The dNTPs in sets or mixes can be purchased from highQu separately.
Taq DNA Polymerase Reference: PCE0202 highQu Taq DNA Polymerase is the classical enzyme for routine PCR applications providing high amplification yields of 3-5 kb targets under various conditions. Taq DNA Polymerase is purified from a recombinant E. coli strain carrying the Taq DNA polymerase gene. Taq DNA polymerase is thermostable 5´ → 3´ DNA polymerase. It lacks 3´ → 5´ exonuclease (proofreading) activity and has low 5´ → 3´ exonuclease activity. Polymerase exhibits deoxynucleotidyl transferase activity resulting in A-overhang at the 3'-ends of PCR products and allowing for TA cloning. The PCR accuracy of Taq DNA Polymerase is 4.5 x 104 (a number of incorporated nucleotides before the first error occurs). The supplied reaction buffer for most flexibility in optimization, contains no dNTPs and no magnesium. The 50 mM MgCl2 solution is provided separately what allows for magnesium optimization starting from 1 mM up to 4 mM final concentration upon the need. The dNTPs in sets or mixes can be purchased from highQu separately.
ALLin™ Hot Start Taq Polymerase Reference: HSE0101 highQu ALLin™ Hot Start Taq Polymerase is the superior sensitive hot-start DNA polymerase. The enzyme activity at room temperature is blocked by a small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation occurs and no background appears. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ Hot Start Taq Mastermix, 2X is available.
ALLin™ Hot Start Taq Polymerase Reference: HSE0105 highQu ALLin™ Hot Start Taq Polymerase is the superior sensitive hot-start DNA polymerase. The enzyme activity at room temperature is blocked by a small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation occurs and no background appears. In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of complex or longer templates and fast cycling. ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 10^4 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors. For the maximum convenience the ALLin™ Hot Start Taq Mastermix, 2X is available.