RTv Reverse Transcriptase Reference: RV101-01 RTv Reverse Transcriptase is obtained from the M-MLV Reverse Transcriptase by directed genetic engineering, which has excellent reverse transcription efficiency, specificity, sensitivity and thermal stability. It is applicable for Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP). With the new generation of hot start technology, the reaction system can be prepared at room temperature. Moreover, the enzyme activity is inhibited at temperatures below 45°C, thus improving specificity.
RTv Reverse Transcriptase (Glycerol-free) Reference: RVL101-01 RTv Reverse Transcriptase (Glycerol-free) is obtained from the M-MLV Reverse Transcriptase by directed genetic engineering, which has excellent reverse transcription efficiency, specificity, sensitivity and thermal stability. It is a lyophilizable version of RTv Reverse Transcriptase (Vazyme #RV101), applicable for Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP). With the new generation of hot start technology, the reaction system can be prepared at room temperature. Moreover, the enzyme activity is inhibited at temperatures below 45°C, thus improving specificity.
Fluorescent LAMP/RT-LAMP Kit Reference: RP711-01 Fluorescent LAMP/RT-LAMP Kit is designed to provide a simple one-step solution for loop-mediated isothermal amplification of DNA or RNA targets. The 2 × Fluorescent LAMP/RT-LAMP Master Mix in this kit contains optimized buffer solution, dNTP, Bst II Pro DNA polymerase and RTv reverse transcriptase for fast and efficient LAMP/RT-LAMP detection. Bst II Pro DNA Polymerase and RTv Reverse Transcriptase use the updated hot start technology, which has excellent specificity and supports the preparation of reaction system at room temperature. This kit provides fluorescent dyes to realize real-time fluorescence detection of LAMP/RT-LAMP.
Fluorescent LAMP/RT-LAMP Kit Reference: RP711-02 Fluorescent LAMP/RT-LAMP Kit is designed to provide a simple one-step solution for loop-mediated isothermal amplification of DNA or RNA targets. The 2 × Fluorescent LAMP/RT-LAMP Master Mix in this kit contains optimized buffer solution, dNTP, Bst II Pro DNA polymerase and RTv reverse transcriptase for fast and efficient LAMP/RT-LAMP detection. Bst II Pro DNA Polymerase and RTv Reverse Transcriptase use the updated hot start technology, which has excellent specificity and supports the preparation of reaction system at room temperature. This kit provides fluorescent dyes to realize real-time fluorescence detection of LAMP/RT-LAMP.
LAMP Fluorescent Dye Reference: RP001-01 LAMP Fluorescent Dye is an intercalating dye with the strong fluorescent signal and little inhibition of the reaction. It is applicable for real-time fluorescent detection of LAMP reactions. This product is a 50 × solution with DMSO as the solvent, compatible with a variety of fluorescent quantitative PCR instruments. It is recommended that the final concentration of the dye be 0.1 - 1×, and please select the SYBR/FAM detection channel when using it.
Taq HS DNA Polymerase Reference: P132-d1 Taq HS DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Taq HS DNA polymerase is still blocked at temperature up to 55°C, which minimizes non-specific amplification during the mixing and system heating. When the reaction is kept at 95°C for more than 30 sec, Champagne Taq antibody is completely inactivated and Taq enzyme activity is completely released, ensuring that the PCR system has extremely high amplification sensitivity and specificity. The activation of Taq HS DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR based on Taq DNA polymerase and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). It is the hot-start Taq enzyme of choice for PCR/qPCR molecular diagnostic reagents. Taq HS DNA polymerase has higher stability and detection rate.
Taq HS DNA Polymerase Reference: P132-d2 Taq HS DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Taq HS DNA polymerase is still blocked at temperature up to 55°C, which minimizes non-specific amplification during the mixing and system heating. When the reaction is kept at 95°C for more than 30 sec, Champagne Taq antibody is completely inactivated and Taq enzyme activity is completely released, ensuring that the PCR system has extremely high amplification sensitivity and specificity. The activation of Taq HS DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR based on Taq DNA polymerase and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). It is the hot-start Taq enzyme of choice for PCR/qPCR molecular diagnostic reagents. Taq HS DNA polymerase has higher stability and detection rate.
Taq HS DNA Polymerase Reference: P132-d3 Taq HS DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Taq HS DNA polymerase is still blocked at temperature up to 55°C, which minimizes non-specific amplification during the mixing and system heating. When the reaction is kept at 95°C for more than 30 sec, Champagne Taq antibody is completely inactivated and Taq enzyme activity is completely released, ensuring that the PCR system has extremely high amplification sensitivity and specificity. The activation of Taq HS DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR based on Taq DNA polymerase and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). It is the hot-start Taq enzyme of choice for PCR/qPCR molecular diagnostic reagents. Taq HS DNA polymerase has higher stability and detection rate.
miRNA 1st Strand cDNA Synthesis Kit (by tailing A) Reference: MR201-01 miRNA 1st strand cDNA Synthesis Kit (by tailing A) is a kit for microRNA (miRNA) reverse transcription (RT) by tailing A. Poly A polymerase (PAP) adds a Poly(A) tail to the 3' end of miRNA or total RNA, and then universal reverse transcription primers initiate RT reaction with reverse transcriptase. HiScript miRNA Enzyme Mix contains bioengineered reverse transcriptase and PAP. Combined with the optimized buffer, the reverse transcription of non-miRNAs is effectively inhibited, achieving highly specific qPCR results. 2 × miRNA RT Mix and HiScript miRNA Enzyme Mix contains all components required for miRNA tailing and reverse transcription. Two reactions can be performed simultaneously in one tube.
BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) Reference: QN222-01 BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) is a probe-based qPCR premix for DNA multiplex amplification, which supports premixing of primers and probes. The BioSmart DNA Polymerase is screened by Vazyme's unique BioSmart platform for enzymes with long sequence of shortest extended bases and strong 3' end mismatch recognition ability. With the high blocking rate dual-species specific antibody (binding to different antigenic epitopes) and an optimized buffer system, it effectively improves multiplex amplification sensitivity, specificity, low-copy detection rate, GC content compatibility and impurity tolerance (blood, ethanol, guanidinium salts, etc.). It supports fast program and primers and probes premixing. In addition, the dUTP/UDG anti-contamination system in 2 × All-Powerful qPCR PreMix effectively prevents aerosol contamination, ensuring accurate qPCR results.
BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) Reference: QN222-02 BioSmart U+ All-Powerful Multiple Probe qPCR PreMix (ONE TUBE) is a probe-based qPCR premix for DNA multiplex amplification, which supports premixing of primers and probes. The BioSmart DNA Polymerase is screened by Vazyme's unique BioSmart platform for enzymes with long sequence of shortest extended bases and strong 3' end mismatch recognition ability. With the high blocking rate dual-species specific antibody (binding to different antigenic epitopes) and an optimized buffer system, it effectively improves multiplex amplification sensitivity, specificity, low-copy detection rate, GC content compatibility and impurity tolerance (blood, ethanol, guanidinium salts, etc.). It supports fast program and primers and probes premixing. In addition, the dUTP/UDG anti-contamination system in 2 × All-Powerful qPCR PreMix effectively prevents aerosol contamination, ensuring accurate qPCR results.
UniPeak U+ One Step RT-qPCR SYBR Green Kit Reference: Q226-01 UniPeak U+ One Step RT-qPCR SYBR Green Kit is a dye-based RT-qPCR reagent specifically designed for quantitative PCR detection using RNA as the template. The dye-based fluorescence quantitative detection presents two major challenges: the difficulty of detecting low-concentration samples and the issue of non-specific amplification. This product addresses these challenges with a next-generation antibody-modified Taq DNA Polymerase and a one-step, temperature-activated Reverse Transcriptase, combined with an optimized buffer system for RT-qPCR, offering enhanced sensitivity and specificity. To further ensure accurate quantification, a dUTP/UDG anti-contamination system is included, eliminating aerosol contamination at room temperature. Additionally, the 5 × gDNA Wiper effectively removes any residual genomic DNA contamination from RNA templates. The 2 × One Step SYBR Green Master Mix includes tracking dye and universal ROX Reference Dye, making it compatible with all types of qPCR instruments, requiring only the addition of primers and templates for amplification.