AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) Reference: Q621-EN01 AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) is a one-tube probe-based RT-qPCR master mix suitable for single or multiple quantitative PCR detection using RNA as a template (such as RNA virus). The enzyme in this master mix uses a hot-start technology based on enzyme modification, with a new generation of high-affinity Taq enzyme antibodies, to achieve efficient blocking and precise release. Combined with one-step dedicated Reverse Transcriptase and optimized buffer, it supports pre-mixing of primers and probes in advance and the mixture can be stored for a long time at low temperature. The sample to be tested can be added directly when using, no additional tube opening/pipetting operation. This product is also compatible with fast programs, which greatly shortens the detection time.
AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) Reference: Q621-EN02 AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) is a one-tube probe-based RT-qPCR master mix suitable for single or multiple quantitative PCR detection using RNA as a template (such as RNA virus). The enzyme in this master mix uses a hot-start technology based on enzyme modification, with a new generation of high-affinity Taq enzyme antibodies, to achieve efficient blocking and precise release. Combined with one-step dedicated Reverse Transcriptase and optimized buffer, it supports pre-mixing of primers and probes in advance and the mixture can be stored for a long time at low temperature. The sample to be tested can be added directly when using, no additional tube opening/pipetting operation. This product is also compatible with fast programs, which greatly shortens the detection time.
ClonExpress Ultra One Step Cloning Kit V2 Reference: C116-01 The ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.
ClonExpress Ultra One Step Cloning Kit V2 Reference: C116-02 The ClonExpress technology is simple, fast, and highly efficient DNA seamless cloning technology. It enables rapid directional cloning of inserts into any site in any vector. Use any method to linearize the vector, and introduce the end sequence of the linearized vector at the 5' end of the insert forward/reverse amplification primer, so that the 5' and 3' ends of the PCR product have the same ends sequence (15 - 20 bp) as the linearized vector, respectively. The PCR product with the same sequence as the end of the vector and the linearized vector are mixed in a certain proportion. Under the catalysis of recombinase, the transformation can be performed at 50°C for 5 - 30 min to complete the directional cloning.ClonExpress Ultra One Step Cloning Kit V2 is a new generation of recombinant cloning kits, compatible with 1 - 5 fragments homologous recombination. Highly optimized 2 × CE Mix further significantly improves the recombination efficiency of multiple fragments. This product has broader compatibility with the GC content of homology arms, which better guarantees the success rate of cloning in more difficult application scenarios.
Ultra-Universal TOPO Cloning Kit Reference: C603-01 Ultra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases the positive rate of cloning.Features - Upgraded version: 4th Generation topoisomerase; higher positive rate and colony counts- Fast: Cloning of either sticky-end or blunt-end DNA in only 5 minutes- Efficient: High efficiency cloning and positive rate>95%- Vector self-ligation inhibitor factor: Effectively prevent vector self-ligation and reduce false positives
Ultra-Universal TOPO Cloning Kit Reference: C603-02 Ultra-Universal TOPO Cloning Kit is the fourth-generation TOPO cloning kit. The kit uses upgraded Topoisomerase with the optimal buffer and has higher enzyme activity. The addition of the second-generation blunting factor and vector self-ligation inhibitor makes this product compatible with both TA cloning and blunt-end cloning, and increases the positive rate of cloning.Features - Upgraded version: 4th Generation topoisomerase; higher positive rate and colony counts- Fast: Cloning of either sticky-end or blunt-end DNA in only 5 minutes- Efficient: High efficiency cloning and positive rate>95%- Vector self-ligation inhibitor factor: Effectively prevent vector self-ligation and reduce false positives
FastPure EndoFree Plasmid Midi Kit Reference: DC205-01 This kit is applicable for extracting bacterial culture that has been cultured overnight in a volume of 15 - 50 ml. It uses an optimized alkaline lysis method to lyse cells and utilizes a unique silica membrane adsorption technique along with special Buffer ERB and Buffer ERW solutions to specifically bind plasmids and effectively remove impurities such as endotoxins, proteins, and genomic DNA. Additionally, the unique P2 solution with indicator can indicate the completeness of lysis and neutralization through color changes, ensuring extraction quality and enabling visual operation. The extracted plasmid DNA has extremely low residual endotoxin levels, making it suitable for various cell transfection and routine operations, including restriction endonuclease digestion, PCR, sequencing, ligation, and other experiments.
Bst II Pro DNA Polymerase Large Fragment Reference: P703-01 Bst II Pro DNA Polymerase Large Fragment is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Compared with Bst II DNA Polymerase Large Fragment (Vazyme #P702), Bst II Pro DNA Polymerase Large Fragment combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymerase activity at temperatures above 50°C. It displays superior amplification speed, specificity, salt tolerance and thermal stability. The reaction system can be prepared at room temperature.
Bst II Pro DNA Polymerase Large Fragment Reference: P703-02 Bst II Pro DNA Polymerase Large Fragment is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Compared with Bst II DNA Polymerase Large Fragment (Vazyme #P702), Bst II Pro DNA Polymerase Large Fragment combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymerase activity at temperatures above 50°C. It displays superior amplification speed, specificity, salt tolerance and thermal stability. The reaction system can be prepared at room temperature.
Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) Reference: PL703-01 Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymeraseactivity at temperatures above 50°C. It displays good amplification speed, high specificity, strong salt tolerance and reliable thermal stability. The reaction system can be prepared at room temperature. This product is a glycerin-free formula, which can be used to develop lyophilized products. It should be noted that excipient ingredients are not included, please add it according to actual requirements.
Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) Reference: PL703-02 Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) is obtained from the large fragment of Bacillus stearothermophilus DNA Polymerase by directed genetic engineering. It contains the 5'→3' polymerase activity and strong strand displacement activity, but lacks 5'→3' exonuclease activity. It is applicable for isothermal amplifications such as LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification) and RCA (Rolling Circle Amplification). Bst II Pro DNA Polymerase Large Fragment (Glycerol-free) combines a new generation of hot start technology to inhibit polymerase activity at temperatures below 50°C and release polymeraseactivity at temperatures above 50°C. It displays good amplification speed, high specificity, strong salt tolerance and reliable thermal stability. The reaction system can be prepared at room temperature. This product is a glycerin-free formula, which can be used to develop lyophilized products. It should be noted that excipient ingredients are not included, please add it according to actual requirements.